An alternative approach to nucleic acid memory
| العنوان: | An alternative approach to nucleic acid memory |
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| المؤلفون: | Christopher Green, Elton Graugnard, Luca Piantanida, William L. Hughes, George D. Dickinson, Golam Md Mortuza, Timothy L. Andersen, Chad S. Watson, Reza M. Zadegan, William Clay, Eric J. Hayden, Wan Kuang |
| المصدر: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Information storage, Computer science, Physics::Instrumentation and Detectors, Science, General Physics and Astronomy, DNA, Single-Stranded, Information Storage and Retrieval, 02 engineering and technology, DNA nanostructures, Proof of Concept Study, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Redundancy (engineering), DNA origami, Nanotechnology, natural sciences, Super-resolution microscopy, Quantitative Biology::Biomolecules, Multidisciplinary, business.industry, Reading (computer), technology, industry, and agriculture, dNaM, General Chemistry, 021001 nanoscience & nanotechnology, Quantitative Biology::Genomics, Nanostructures, 030104 developmental biology, chemistry, Computer data storage, Nucleic acid, Nucleic Acid Conformation, 0210 nano-technology, business, Error detection and correction, DNA, Computer hardware, Algorithms, DNA computing and cryptography |
| الوصف: | DNA is a compelling alternative to non-volatile information storage technologies due to its information density, stability, and energy efficiency. Previous studies have used artificially synthesized DNA to store data and automated next-generation sequencing to read it back. Here, we report digital Nucleic Acid Memory (dNAM) for applications that require a limited amount of data to have high information density, redundancy, and copy number. In dNAM, data is encoded by selecting combinations of single-stranded DNA with (1) or without (0) docking-site domains. When self-assembled with scaffold DNA, staple strands form DNA origami breadboards. Information encoded into the breadboards is read by monitoring the binding of fluorescent imager probes using DNA-PAINT super-resolution microscopy. To enhance data retention, a multi-layer error correction scheme that combines fountain and bi-level parity codes is used. As a prototype, fifteen origami encoded with ‘Data is in our DNA!\n’ are analyzed. Each origami encodes unique data-droplet, index, orientation, and error-correction information. The error-correction algorithms fully recover the message when individual docking sites, or entire origami, are missing. Unlike other approaches to DNA-based data storage, reading dNAM does not require sequencing. As such, it offers an additional path to explore the advantages and disadvantages of DNA as an emerging memory material. Encoding data in DNA is a promising approach to high density data storage. Here the authors present a prototype sequencing-free method that uses the spatial orientation of DNA strands with super-resolution microscopy readout. |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5306a89dbf77f8a7477ec6a1ecdfe7f4Test https://pubmed.ncbi.nlm.nih.gov/33888693Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5306a89dbf77f8a7477ec6a1ecdfe7f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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