Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity
| العنوان: | Imaging Tumorous Methylglyoxal by an Activatable Near-Infrared Fluorescent Probe for Monitoring Glyoxalase 1 Activity |
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| المؤلفون: | Ruimin Huang, Zhiai Xu, Wen Zhang, Yijing Dang, Ao Zhang, Yi Lai, Lingling Li, Haijun Yu, Fengyang Wang, Chunyong Ding, Yi Luo |
| المصدر: | Analytical Chemistry. 91:15577-15584 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | In situ, Infrared Rays, Mice, Nude, Apoptosis, Breast Neoplasms, Tumor initiation, 010402 general chemistry, 01 natural sciences, Analytical Chemistry, Mice, chemistry.chemical_compound, In vivo, Sense (molecular biology), Tumor Cells, Cultured, Animals, Humans, Enzyme Inhibitors, Cell Proliferation, Fluorescent Dyes, Detection limit, Mice, Inbred BALB C, Chemistry, 010401 analytical chemistry, Methylglyoxal, Near-infrared spectroscopy, Lactoylglutathione Lyase, Pyruvaldehyde, Xenograft Model Antitumor Assays, Fluorescence, 0104 chemical sciences, Biophysics, Female |
| الوصف: | The accurate detection of tumorous methylglyoxal (MGO) and its detoxifier glyoxalase 1 (GLO1) in living systems is critical for understanding their roles in tumor initiation and progression. To date, the in situ fluorescence detection of endogenous MGO and GLO1 in tumor has not been reported. Herein we developed a near-infrared (NIR) fluorescent probe MEBTD to specifically detect tumorous MGO. Compared with previously reported MGO fluorescent probes, MEBTD exhibits several distinct advantages, including NIR emission, high selectivity with an MGO detection limit of 18 nM, and a 131-fold off-on ratio. The probe could sense GLO1 activity and monitor the therapeutic effect of GLO1 inhibitors by imaging tumorous MGO in a both a real-time and in situ manner, demonstrating that the biological effect of GLO1 inhibitors is dependent on the GLO1 activity. Furthermore, MEBTD enables the visualization of tumorous MGO induced by GLO1 inhibitors in vivo. To the best of our knowledge, MEBTD is the first NIR fluorescent probe for specifically imaging tumorous MGO in living animals, indicating the promising potential for tumor diagnosis and therapeutic evaluation. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff1aa70df20110e89ffdc6531abed29Test https://doi.org/10.1021/acs.analchem.9b03600Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4ff1aa70df20110e89ffdc6531abed29 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
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