The role of extracellular vesicles from stored RBC units in B lymphocyte survival and plasma cell differentiation
| العنوان: | The role of extracellular vesicles from stored RBC units in B lymphocyte survival and plasma cell differentiation |
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| المؤلفون: | Ruiqin Hou, Ying Yang, Hui Tan, Haiqiang Jin, Jia Wu, Yan Wang, Yuqing Wang, Jianhua Zhang, Wenqin Tian, Yuhan Gao |
| المصدر: | Journal of Leukocyte Biology. 108:1765-1776 |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Lipopolysaccharides, 0301 basic medicine, Erythrocytes, Lymphocyte, Plasma Cells, Immunology, Mice, SCID, Biology, Plasma cell, Extracellular Vesicles, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Mice, Inbred NOD, In vivo, Plasma cell differentiation, medicine, Animals, Immunology and Allergy, Platelet, B cell, Cell Differentiation, Cell Biology, Extracellular vesicle, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Blood Preservation, 030220 oncology & carcinogenesis, Interferon Regulatory Factors, Positive Regulatory Domain I-Binding Factor 1 |
| الوصف: | Extracellular vesicles (EVs) are small, double-membrane vesicles derived from erythrocytes, leukocytes, platelets, and cells of multiple tissues under physiologic or pathologic conditions. The role of EVs in stored RBC units is of great interest with respect to transfusion-related immunomodulation. The current study focuses on the quantity of EVs isolated from stored RBC units and their action on B cell-mediated immune responses. The in vitro experiment demonstrated that EVs exhibited a negative role in B cell survival, plasmacytic differentiation, and class switch recombination under LPS stimulation. Furthermore, LPS-induced antibody production was significantly decreased after EVs injection in vivo. Biochemical analysis revealed that EVs hampered the expression of Blimp-1 and IRF4 and the activation of NF-κB pathway in LPS-primed B cells. Overall, these data imply a vital role for EVs isolated from RBC units in B cell-mediated immune responses. |
| تدمد: | 1938-3673 0741-5400 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f66a2c259ac79274285ef6fcecfa8d3Test https://doi.org/10.1002/jlb.1a0220-666rTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4f66a2c259ac79274285ef6fcecfa8d3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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