Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy
| العنوان: | Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy |
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| المؤلفون: | Roberta Bortul, Marina Zweyer, Ines Delfino, Laura Andolfi, Stefania Luppi, Elena Masiero, Giuseppe Ricci, Elena Giolo, Marco Lazzarino, Simone Dal Zilio, Monica Martinelli |
| المساهمون: | Andolfi, Laura, Masiero, Elena, Giolo, Elena, Martinelli, Monica, Luppi, Stefania, DAL ZILIO, Simone, Delfino, Ine, Bortul, Roberta, Zweyer, Marina, Ricci, Giuseppe, Lazzarino, Marco |
| المصدر: | Integrative biology (Camb., Print) 8 (2016): 886–893. doi:10.1039/c6ib00044d info:cnr-pdr/source/autori:Andolfi L.; Masiero E.; Giolo E.; Martinelli M.; Luppi S.; Dal Zilio S.; Delfino I.; Bortul R.; Zweyer M.; Ricci G.; Lazzarino M./titolo:Investigating the mechanical properties of zona pellucida of whole human oocytes by atomic force spectroscopy/doi:10.1039%2Fc6ib00044d/rivista:Integrative biology (Camb., Print)/anno:2016/pagina_da:886/pagina_a:893/intervallo_pagine:886–893/volume:8 |
| بيانات النشر: | Royal Society of Chemistry, Cambridge , Regno Unito, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, medicine.medical_treatment, zona pellucida, Biophysics, atomic force spectroscopy, Cell Separation, Fertilization in Vitro, mechanical properties, Biology, Microscopy, Atomic Force, Biochemistry, ICSI, Intracytoplasmic sperm injection, Andrology, 03 medical and health sciences, Human fertilization, cell mechanics, Pregnancy, medicine, cell elasticity, Humans, oocytes, mechanical propertie, Sperm Injections, Intracytoplasmic, Zona pellucida, oocyte, reproductive and urinary physiology, In vitro fertilisation, Metaphase ii, urogenital system, human oocytes, Force spectroscopy, Anatomy, Oocyte, Spermatozoa, Elasticity, 030104 developmental biology, medicine.anatomical_structure, embryonic structures, Female, Metaphase i, Stress, Mechanical |
| الوصف: | The role of mechanics in numerous biological processes is nowadays recognized, while in others, such as the fertilization process, it is still neglected. In the case of oocytes the description of their mechanical properties could improve the comprehension of the oocyte-spermatozoon interaction and be helpful for application in in vitro fertilization (IVF) clinics. Herein the mechanical properties of whole human oocytes (HOs) immediately after retrieval are investigated by indentation measurements with atomic force spectroscopy under physiological conditions. Measurements are performed on immature (metaphase I - MI) and mature (metaphase II - MII) HOs. According to their morphological characteristics MII-HOs are classified as "suitable" and "rejected"; these latter would be usually rejected for intracytoplasmic sperm injection (ICSI). For all maturation stages we observe that the elastic response of the zona pellucida (ZP) outer layer was different and distinguishable from the rest of the ZP-HO. The elasticity of this ZP outer layer varies with maturation and quality: stiffness decreases from immature MI to good quality MII, up to poor-quality rejected MII. An indirect analysis with IVF outcome indicates that the ZP outer layer of analysed HOs donated by women who achieved pregnancy is stiffer than that of HOs from women with negative outcome. Our findings suggest that mechanical properties can represent important oocyte quality indicators that may be exploited for the design of innovative ICSI dedicated cell sorters. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d2e446af5bc5661b16586fb7287ea22Test http://www.cnr.it/prodotto/i/435694Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4d2e446af5bc5661b16586fb7287ea22 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |