Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice
| العنوان: | Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice |
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| المؤلفون: | Mingyuan Zhang, Ouyang Yiqiang, Jinning Liang, Bing Hu, Sun Junming, He Min, Mo Zhongxiang, Lichao Yang, Jiajuan Liu, Xiaoping Guo, Xuejing Huang, Ping Yu |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | John Wiley and Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, Myosin light-chain kinase, Myosin Light Chains, Blotting, Western, Cardiomegaly, Sudden death, Muscle hypertrophy, Transcriptome, Mice, Downregulation and upregulation, Internal medicine, MYL7, medicine, Animals, Myocytes, Cardiac, Vasorin, business.industry, cardiac hypertrophy, Gene Expression Profiling, Membrane Proteins, Cell Biology, Original Articles, deficiency, Up-Regulation, Blot, Endocrinology, Molecular Medicine, Immunohistochemistry, Original Article, business, Apoptosis Regulatory Proteins |
| الوصف: | Vasorin (VASN) is an important transmembrane protein associated with development and disease. However, it is not clear whether the death of mice with VASN deficiency (VASN −/−) is related to cardiac dysfunction. The aim of this research was to ascertain whether VASN induces pathological cardiac hypertrophy by targeting myosin light chain 7 (MYL7). VASN −/− mice were produced by CRISPR/Cas9 technology and inbreeding. PCR amplification, electrophoresis, real‐time PCR and Western blotting were used to confirm VASN deficiency. Cardiac hypertrophy was examined by blood tests, histological analysis and real‐time PCR, and key downstream factors were identified by RNA sequencing and real‐time PCR. Western blotting, immunohistochemistry and electron microscopy analysis were used to confirm the downregulation of MYL7 production and cardiac structural changes. Our results showed that sudden death of VASN −/− mice occurred 21–28 days after birth. The obvious increases in cardiovascular risk, heart weight and myocardial volume and the upregulation of hypertrophy marker gene expression indicated that cardiac hypertrophy may be the cause of death in young VASN −/− mice. Transcriptome analysis revealed that VASN deficiency led to MYL7 downregulation, which induced myocardial structure abnormalities and disorders. Our results revealed a pathological phenomenon in which VASN deficiency may lead to cardiac hypertrophy by downregulating MYL7 production. However, more research is necessary to elucidate the underlying mechanism. |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b0052b3548e60d12a2656e291ea2193Test http://europepmc.org/articles/PMC8742182Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b0052b3548e60d12a2656e291ea2193 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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