Hydrogen‑deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints
| العنوان: | Hydrogen‑deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints |
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| المؤلفون: | Francesca Galluzzi, Stéphane Chaignepain, Julie Arslanoglu, Caroline Tokarski |
| المساهمون: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), European Project: H2020 MSCA-60 ITN |
| المصدر: | Biophysical Chemistry Biophysical Chemistry, 2022, 289, ⟨10.1016/j.bpc.2022.106861⟩ |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Lead, Protein Conformation, Organic Chemistry, Paint, Biophysics, Deuterium Exchange Measurement, Proteins, Hydrogen Deuterium Exchange-Mass Spectrometry, [CHIM.MATE]Chemical Sciences/Material chemistry, Deuterium, Biochemistry |
| الوصف: | Little is known about structural alterations of proteins within the polymeric films of paints. For the first time, hydrogen‑deuterium exchange mass spectrometry (HDX-MS) was implemented to explore the conformational alterations of proteins resulting from their interaction with inorganic pigments within the early stages of the paint film formation. Intact protein analysis and bottom-up electrospray-ionisation mass spectrometry strategies combined with progressively increasing deuterium incubation times were used to compare the protein structures of the model protein hen egg-white lysozyme (HEWL) extracted from newly dried non-pigmented films and newly dried films made from a freshly made mixture of HEWL with lead white pigment (2PbCO3 Pb(OH)2). The action of other pigments was also investigated, expanding the HDX study with a global approach to paint models of HEWL mixed with zinc white (ZnO), cinnabar (HgS) and red lead (Pb3O4) pigments. The results show structural modifications of HEWL induced by the interaction with the pigment metal ions during the paint formulation after drying and prior to ageing. Both the charge distribution of HEWL proteoforms, its oxidation rate and its deuterium absorption rate, were influenced by the pigment type, providing the first insights into the correlation of pigment type/metal cation to specific chemistries related to protein stability. |
| تدمد: | 1873-4200 0301-4622 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4af0aab181eaef42b346c4a2403f93deTest https://pubmed.ncbi.nlm.nih.gov/35940022Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....4af0aab181eaef42b346c4a2403f93de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18734200 03014622 |
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