Prognostic significance of RACGAP1 mRNA expression in high-risk early breast cancer: a study in primary tumors of breast cancer patients participating in a randomized Hellenic Cooperative Oncology Group trial
| العنوان: | Prognostic significance of RACGAP1 mRNA expression in high-risk early breast cancer: a study in primary tumors of breast cancer patients participating in a randomized Hellenic Cooperative Oncology Group trial |
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| المؤلفون: | Pliarchopoulou, K., Kalogeras, K. T., Kronenwett, R., Wirtz, R. M., Eleftheraki, A. G., Batistatou, Anna, Bobos, M., Soupos, N., Polychronidou, G., Gogas, H., Samantas, E., Christodoulou, C., Makatsoris, T., Pavlidis, Nicholas, Pectasides, Dimitrios, Fountzilas, George |
| المساهمون: | Pavlidis, Nicholas [0000-0002-2195-9961] |
| المصدر: | Cancer chemotherapy and pharmacology |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Oncology, Survival rate, Scoring system, Messenger rna, Cell, Toxicology, Breast cancer, Antineoplastic Combined Chemotherapy Protocols, Pharmacology (medical), Rac gtpase activating protein 1 gene, Ki-67 antigen, Quantitative analysis, Disease free survival, Survival time, Gene expression regulation, GTPase-Activating Proteins, Prognosis, Gene Expression Regulation, Neoplastic, Survival Rate, Retrospective study, Paclitaxel, Reverse transcription polymerase chain reaction, Regression Analysis, Human, medicine.medical_specialty, Major clinical study, Nottingham prognostic index, Disease-Free Survival, Article, Cancer grading, Randomized controlled trials as topic, Disease association, Humans, RNA, Messenger, Cyclophosphamide, Retrospective Studies, Aged, Pharmacology, Cancer prognosis, Patient Selection, medicine.disease, Retrospective studies, Drug effect, Ki-67 Antigen, Methotrexate, Gene identification, chemistry, Protein expression, Risk factor, Gene expression, Breast neoplasms, Cancer Research, Unclassified drug, Messenger, Metastasis, Qrt-pcr, Cancer risk, chemistry.chemical_compound, Randomized controlled trial (topic), Patient selection, Guanosine triphosphatase activating protein, Overall survival, Gtpase-activating proteins, Middle aged, Reverse transcriptase polymerase chain reaction, Randomized Controlled Trials as Topic, Priority journal, Risk assessment, Reverse Transcriptase Polymerase Chain Reaction, Middle Aged, Primary tumor, medicine.anatomical_structure, Real-time polymerase chain reaction, Nottingham Prognostic Index, Female, Fluorouracil, Protein determination, Regression analysis, Prognostic value, Ki67, Epirubicin, medicine.drug, Adult, Proportional hazards models, Disease-free survival, Histopathology, Breast Neoplasms, Young Adult, Antineoplastic combined chemotherapy protocols, Internal medicine, medicine, Early cancer, Human tissue, Racgap1, Oncogene, Proportional Hazards Models, Neoplastic, business.industry, Cancer survival, High risk patient, Young adult, Multivariate analysis, Rac gtpase activating protein 1, Multivariate Analysis, Rna, Ki 67 antigen, Prediction, business, Controlled study, Gene function |
| الوصف: | Purpose: RACGAP1 is a Rac GTPase-activating protein involved in cell growth regulation, cell transformation and metastasis. The aim of the present study was to explore the prognostic and/or predictive significance of RACGAP1 mRNA expression on disease-free survival (DFS) and overall survival (OS) in high-risk early breast cancer patients and compare it to that of Ki67 protein expression and to the Nottingham prognostic index (NPI). Methods: A total of 595 high-risk breast cancer patients were treated in a two-arm trial evaluating postoperative dose-dense sequential chemotherapy with epirubicin followed by CMF with or without paclitaxel. RNA was extracted from 314 formalin-fixed paraffin-embedded primary tumor tissue samples followed by one-step quantitative RT-PCR for assessing RACGAP1 mRNA expression. Results: High RACGAP1 mRNA expression (above the median) was associated with poor DFS (log-rank, p = 0.002) and OS (p < 0.001). High histological grade, as well as high Ki67 protein expression, was more frequent in the high-expression group of RACGAP1. Results of the Cox multivariate regression analysis revealed that high RACGAP1 mRNA expression independently predicted poor overall survival (Wald's p = 0.008). High Ki67 protein expression was also an adverse prognostic factor for death (p = 0.016), while high NPI score values were not. Conclusions: High RACGAP1 mRNA expression, as assessed by qRT-PCR, was found to be of adverse prognostic significance in high-risk early breast cancer patients treated with dose-dense sequential chemotherapy. The utility of RACGAP1 mRNA expression in patient selection for treatment with aggressive chemotherapy regimens should be further explored and validated in larger cohorts. © 2012 Springer-Verlag Berlin Heidelberg. 71 1 245 255 |
| تدمد: | 1432-0843 0344-5704 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aeaf136db5f1bc63a52f742e8828fd7Test https://doi.org/10.1007/s00280-012-2002-zTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4aeaf136db5f1bc63a52f742e8828fd7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320843 03445704 |
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