Light/heat effects on RNA editing in chloroplast NADH-plastoquinone oxidoreductase subunit 2 (ndhB) gene of Calotropis (Calotropis procera)
| العنوان: | Light/heat effects on RNA editing in chloroplast NADH-plastoquinone oxidoreductase subunit 2 (ndhB) gene of Calotropis (Calotropis procera) |
|---|---|
| المؤلفون: | Ahmed M. Ramadan |
| المصدر: | Journal of Genetic Engineering and Biotechnology, Vol 18, Iss 1, Pp 1-8 (2020) Journal of Genetic Engineering & Biotechnology |
| بيانات النشر: | SpringerOpen, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, RNA editing, Desert plant, Light, Calotropis (Calotropis procera), lcsh:QH426-470, lcsh:Biotechnology, Plastoquinone, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Calotropis procera, Complementary DNA, lcsh:TP248.13-248.65, Genetics, Coding region, ndhB, Gene, biology, Research, Calotropis, biology.organism_classification, Chloroplast, lcsh:Genetics, 030104 developmental biology, chemistry, Biochemistry, 010606 plant biology & botany, Biotechnology |
| الوصف: | Background RNA editing is common in terrestrial plants, especially in mitochondria and chloroplast. In the photosynthesis process, NAD dehydrogenase plays a very important role. Subunit 2 of NADH-dehydrogenase is one of the major subunits in NAD dehydrogenase complex. Using desert plant Calotropis (Calotropis procera), this study focuses on the RNA editing activity of ndhB based on light time. Results NdhB (NADH-dehydrogenase subunit 2) gene accession no. MK144329 was isolated from Calotropis procera genomic data (PRJNA292713). Additionally, using RNA-seq data, the cDNA of the ndhB gene of C. procera was isolated at three daylight periods, i.e., dawn (accession no. MK165161), at midday (accession no. MK165160), and pre-dusk (accession no. MK165159). Seven RNA editing sites have been found in several different positions (nucleotide no. C467, C586, C611, C737, C746, C830, and C1481) within the ndhB coding region. The rate of these alterations was deferentially edited across the three daylight periods. RNA editing rate of ndhB gene was highest at dawn, (87.5, 79.6, 78.5, 76, 68.6, 39.3, and 96.9%, respectively), less in midday (74.8, 54.1, 62.6, 47.4, 45.5, 47.4, and 93.4%, respectively), and less at pre-dusk (67, 52.6, 56.9, 40.1, 40.7, 33.2, and 90%, respectively), also all these sites were validated by qRT-PCR. Conclusion The differential editing of chloroplast ndhB gene across light periods may be led to a somehow relations between the RNA editing and control of photosynthesis. |
| اللغة: | English |
| تدمد: | 2090-5920 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a7a363c24f2a30a4c66c14a4c27672cTest http://link.springer.com/article/10.1186/s43141-020-00064-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4a7a363c24f2a30a4c66c14a4c27672c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20905920 |
|---|