Evidence of Two Distinct Subsubtypes within the HIV-1 Subtype A Radiation
| العنوان: | Evidence of Two Distinct Subsubtypes within the HIV-1 Subtype A Radiation |
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| المؤلفون: | Gao, F., Vidal, N., Li, Y., Trask, S. A., Chen, Y., Kostrikis, Leontios G., Ho, David D., Kim, J., Oh, M. -D, Choe, K., Salminen, M., Robertson, D. L., Shaw, G. M., Hahn, B. H., Peeters, M. |
| المساهمون: | Kostrikis, Leontios G. [0000-0002-5340-7109] |
| المصدر: | AIDS Research and Human Retroviruses AIDS Res.Hum.Retroviruses |
| بيانات النشر: | Mary Ann Liebert Inc, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | virus strain, molecular cloning, sequence analysis, genotype, polymerase chain reaction, Lineage (evolution), Human immunodeficiency virus 1, HIV Infections, phylogeny, data base, mononuclear cell, Genotype, Epidemiology, Molecular, correlation function, virus classification, Clade, Phylogeny, Genetics, Molecular Epidemiology, clinical article, Phylogenetic tree, adult, cell DNA, article, Nucleic acid sequence, Genes, gag, Genes, pol, female, Infectious Diseases, priority journal, Democratic Republic of the Congo, virus typing, nomenclature, phenotype, Sequence analysis, Molecular Sequence Data, Immunology, Genome, Viral, Biology, Genes, env, Human immunodeficiency virus infection, Phylogenetics, Virology, geographic distribution, Humans, controlled study, human, Korea, Molecular epidemiology, human cell, nucleotide sequence, amino acid sequence, Genes, nef, radiation, virus genome, Cyprus, HIV-1 |
| الوصف: | Members of HIV-1 group M are responsible for the vast majority of AIDS cases worldwide and have been classified on the basis of their phylogenetic relationships into nine roughly equidistant clades, termed subtypes. Although there are no known phenotypic correlates for these genotypes, the disproportionate spread of certain of these lineages has been taken to indicate that subtype-specific biological differences may exist. The subtype nomenclature thus remains an important molecular epidemiological tool with which to track the course of the group M pandemic. In this study, we have characterized HIV-1 strains described previously as unusual subtype A variants on the basis of partial sequence analysis. Six such strains from Cyprus (CY), South Korea (KR), and the Democratic Republic of Congo (CD) were PCR amplified from infected cell culture or patient PBMC DNA, cloned, and sequences in their entirety (94CY017, 97KR004, 97CDKTB48, and 97CDKP58) or as half genomes (97CDKS10 and 97CDKFE4). Distance and phylogenetic analyses showed that four of these viruses (94CY017, 97CDKTB48, 97CDKFE4, and 97CDKS10) were closely related to each other, but quite divergent from all other HIV-1 strains, except for subtype A viruses, which represented their closest relatives. In phylogenetic trees from gag, pol, env, and nef regions, the four newly characterized HIV-1 strains formed a distinct sister clade to subtype A, which was as closely related to subtype A as subsubtypes F1 and F2 are to each other. According to current nomenclature rules, this defines a subsubtype, which we have tentatively termed A2. The two other viruses, 97KR004 and 97CDKP58, as well as a full-length HIV-1 sequence from the sequence database (ZAM184), were found to represent complex A2/D, A2/G, and A2/C recombinants, respectively. These results indicate that HIV-1 subtype A is composed of two subsubtypes (A1 and A2), both of which appear to have a widespread geographic distribution. The A2 viruses described here represent the first reference reagents for this new group M lineage. 17 675 688 Cited By :66 |
| تدمد: | 1931-8405 0889-2229 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46d39fc9204505431419918d9a00fd64Test https://doi.org/10.1089/088922201750236951Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46d39fc9204505431419918d9a00fd64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19318405 08892229 |
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