Tracing the cellular basis of islet specification in mouse pancreas
| العنوان: | Tracing the cellular basis of islet specification in mouse pancreas |
|---|---|
| المؤلفون: | Shosei Yoshida, Lemonia Chatzeli, Magdalena K. Sznurkowska, Roberta Azzarelli, Edouard Hannezo, Tatsuro Ikeda, Benjamin D. Simons, Anna Philpott |
| المساهمون: | Hannezo, Edouard [0000-0001-6005-1561], Azzarelli, Roberta [0000-0002-8160-7538], Ikeda, Tatsuro [0000-0002-8503-8096], Yoshida, Shosei [0000-0001-8861-1866], Philpott, Anna [0000-0003-3789-2463], Simons, Benjamin D. [0000-0002-3875-7071], Apollo - University of Cambridge Repository, Simons, Benjamin D [0000-0002-3875-7071] |
| المصدر: | Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Nature Communications |
| بيانات النشر: | Nature Publishing Group UK, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cellular basis, Male, Cell division, endocrine system diseases, Cellular differentiation, Organogenesis, General Physics and Astronomy, 631/136/2060, Mice, 0302 clinical medicine, 631/114/2397, Genes, Reporter, 631/136/142, Insulin-Secreting Cells, Computational models, 14/19, lcsh:Science, Multidisciplinary, geography.geographical_feature_category, Microscopy, Confocal, Stem Cells, article, Cell Differentiation, Islet, Cell biology, medicine.anatomical_structure, Differentiation, Models, Animal, Female, 64/60, endocrine system, Science, Morphogenesis, Embryonic Development, Mice, Transgenic, Biology, Models, Biological, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Imaging, Three-Dimensional, medicine, Animals, Cell Lineage, Computer Simulation, Progenitor cell, Pancreas, geography, Pancreatic islets, General Chemistry, Embryo, Mammalian, Embryonic stem cell, Luminescent Proteins, 030104 developmental biology, Glucagon-Secreting Cells, 13/51, 14/63, lcsh:Q, 030217 neurology & neurosurgery |
| الوصف: | Pancreatic islets play an essential role in regulating blood glucose level. Although the molecular pathways underlying islet cell differentiation are beginning to be resolved, the cellular basis of islet morphogenesis and fate allocation remain unclear. By combining unbiased and targeted lineage tracing, we address the events leading to islet formation in the mouse. From the statistical analysis of clones induced at multiple embryonic timepoints, here we show that, during the secondary transition, islet formation involves the aggregation of multiple equipotent endocrine progenitors that transition from a phase of stochastic amplification by cell division into a phase of sublineage restriction and limited islet fission. Together, these results explain quantitatively the heterogeneous size distribution and degree of polyclonality of maturing islets, as well as dispersion of progenitors within and between islets. Further, our results show that, during the secondary transition, α- and β-cells are generated in a contemporary manner. Together, these findings provide insight into the cellular basis of islet development. The cellular basis of islet morphogenesis and fate allocation remain unclear. Here, the authors use a R26-CreER-R26R-Confetti mouse line to follow quantitatively the clonal dynamics of islet formation showing how, during the secondary transition, islet progenitors amplify through rounds of stochastic cell division before becoming restricted to α and β cell sublineages. |
| وصف الملف: | application/pdf; text/xml; application/zip |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46bdba4fc1343e37926a805c31f3c0e4Test https://www.repository.cam.ac.uk/handle/1810/311182Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46bdba4fc1343e37926a805c31f3c0e4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |