circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis
| العنوان: | circANRIL reduces vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis |
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| المؤلفون: | Hongling Ji, Rui Guo, Jian Yang, Peixia Shi, Huajuan Zhang, Jianwei Wang |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | D.A. Spandidos, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, medicine.medical_specialty, Inflammation, medicine.disease_cause, Coronary artery disease, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), Internal medicine, Lactate dehydrogenase, Medicine, oxidative stress, Glyceraldehyde 3-phosphate dehydrogenase, Coronary atherosclerosis, biology, business.industry, vascular endothelial injury, General Medicine, Articles, medicine.disease, Malondialdehyde, 030104 developmental biology, Endocrinology, chemistry, inflammation, 030220 oncology & carcinogenesis, biology.protein, medicine.symptom, business, circANRIL, Oxidative stress, coronary artery disease |
| الوصف: | Effects of circular antisense non-coding RNA in the INK4 locus (circANRIL) on vascular endothelial injury, oxidative stress and inflammation in rats with coronary atherosclerosis were studied by establishing a rat model of coronary atherosclerosis in which circANRIL was differentially expressed. A total of 40 healthy Sprague Dawley (SD) rats were randomly divided into research group (n=32) and control group (n=8). In research group, a rat model of coronary atherosclerosis was established without special treatment. The blood calcium (Ca2+) and lipid levels in the two groups were compared. After cell transfection, the rats were divided into blank group (untransfected), negative group (transfected with blank vector), circANRIL group (transfected with circANRIL overexpression plasmid) and circANRIL inhibitor group (transfected with circANRIL silencer). Then the levels of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in each group were compared. Western blotting was adopted to detect the expressions of phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK), p38MAPK and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Finally, p-p38MAPK/GAPDH, p38MAPK/GAPDH and p-p38MAPK/p38MAPK were calculated. There were significant differences in the levels of serum Ca2+ and lipid between control group and research group (P |
| اللغة: | English |
| تدمد: | 1792-1015 1792-0981 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4694155a4941a7a239fe1dd0652120eaTest http://europepmc.org/articles/PMC7401493Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4694155a4941a7a239fe1dd0652120ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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