Transitional correlation between inner-membrane potential and ATP levels of neuronal mitochondria
| العنوان: | Transitional correlation between inner-membrane potential and ATP levels of neuronal mitochondria |
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| المؤلفون: | Kotaro Oka, R. Suzuki, Kohji Hotta |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Growth Cones, lcsh:Medicine, Mitochondrion, Mitochondrial Size, Axonal Transport, Mitochondrial Dynamics, Article, 03 medical and health sciences, Adenosine Triphosphate, Ganglia, Spinal, Animals, Inner membrane, Premovement neuronal activity, Rats, Wistar, Inner mitochondrial membrane, lcsh:Science, Cells, Cultured, Membrane Potential, Mitochondrial, Neurons, Membrane potential, Multidisciplinary, Chemistry, lcsh:R, Axons, Mitochondria, Rats, 030104 developmental biology, Mitochondrial Membranes, Biophysics, Axoplasmic transport, Mitochondrial fission, lcsh:Q, sense organs, Signal Transduction |
| الوصف: | The importance of highly active mitochondria and their contribution to neuronal function has been of recent interest. In most cases, however, mitochondrial activity is estimated using measurements of mitochondrial inner membrane potential (IMPmito), and little is known about the dynamics of native mitochondrial ATP (ATPmito). This study conducted simultaneous imaging of IMPmito and ATPmito in neurons to explore their behaviour and their correlation during physiological mitochondrial/neuronal activity. We found that mitochondrial size, transport velocity and transport direction are not dependent on ATPmito or IMPmito. However, changes in ATPmito and IMPmito during mitochondrial fission/fusion were found; IMPmito depolarized via mitochondrial fission and hyperpolarized via fusion, and ATPmito levels increased after fusion. Because the density of mitochondria is higher in growth cones (GCs) than in axonal processes, integrated ATPmito signals (density × ATPmito) were higher in GCs. This integrated signal in GCs correlated with axonal elongation. However, while the averaged IMPmito was relatively hyperpolarized in GCs, there was no correlation between IMPmito in GCs and axonal elongation. A detailed time-course analysis performed to clarify the reason for these discrepancies showed that IMPmito and ATPmito levels did not always correlate accurately; rather, there were several correlation patterns that changed over time. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4590030260d0da7732d7c86e4cabed40Test http://link.springer.com/article/10.1038/s41598-018-21109-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4590030260d0da7732d7c86e4cabed40 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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