High-throughput microcircuit analysis of individual human brains through next-generation multineuron patch-clamp
| العنوان: | High-throughput microcircuit analysis of individual human brains through next-generation multineuron patch-clamp |
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| المؤلفون: | Jörg R. P. Geiger, Henrike Planert, Franz Xaver Mittermaier, Henrik Alle, Ulf C. Schneider, Yangfan Peng |
| المصدر: | eLife eLife, Vol 8 (2019) |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Patch-Clamp Techniques, Mouse, QH301-705.5, Computer science, Science, Cortical slice, General Biochemistry, Genetics and Molecular Biology, Software, Automated patch clamp, Humans, Patch clamp, Biology (General), Throughput (business), microcircuit, Neurons, General Immunology and Microbiology, Subthreshold conduction, business.industry, General Neuroscience, multipatch, Pipette, Brain, Usability, Small sample, General Medicine, human physiology, Tools and Resources, connectivity, Medicine, Rat, Nerve Net, business, automated patch-clamp, human cortex, Neuroscience, Human |
| الوصف: | Comparing neuronal microcircuits across different brain regions, species and individuals can reveal common and divergent principles of network computation. Simultaneous patch-clamp recordings from multiple neurons offer the highest temporal and subthreshold resolution to analyse local synaptic connectivity. However, its establishment is technically complex and the experimental performance is limited by high failure rates, long experimental times and small sample sizes. We introduce an in-vitro multipatch setup with an automated pipette pressure and cleaning system facilitating recordings of up to 10 neurons simultaneously and sequential patching of additional neurons. We present hardware and software solutions that increase the usability, speed and data throughput of multipatch experiments which allowed probing of 150 synaptic connections between 17 neurons in one human cortical slice and screening of over 600 connections in tissue from a single patient. This method will facilitate the systematic analysis of microcircuits and allow unprecedented comparisons at the level of individuals. |
| تدمد: | 2050-084X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::455ff15bf59cc2957b125ea55e0bbbcdTest https://doi.org/10.7554/elife.48178Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....455ff15bf59cc2957b125ea55e0bbbcd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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