PD‑L1/PD‑1 axis serves an important role in natural killer cell‑induced cytotoxicity in osteosarcoma
| العنوان: | PD‑L1/PD‑1 axis serves an important role in natural killer cell‑induced cytotoxicity in osteosarcoma |
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| المؤلفون: | Li Chen, Ming‑Lei Zhang, Da‑Liang Kong, Yan‑Jiao Li |
| المصدر: | Oncology Reports. |
| بيانات النشر: | Spandidos Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Cytotoxicity, Immunologic, Male, 0301 basic medicine, Cancer Research, Programmed Cell Death 1 Receptor, Cell, Apoptosis, Bone Neoplasms, B7-H1 Antigen, Granzymes, Natural killer cell, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, medicine, Humans, Cytotoxicity, Osteosarcoma, Dose-Response Relationship, Drug, biology, Chemistry, General Medicine, Cell cycle, medicine.disease, Killer Cells, Natural, Granzyme B, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, biology.protein, Cancer research, Antibody |
| الوصف: | Osteosarcoma is a serious malignancy in pediatric patients, which comprises 2.4% of fatal cancer in children and achieves 20% of all primary bone cancers. In the present study, we employed three human osteosarcoma cell lines MG‑63, HOS and U2OS for susceptibility to cytolytic activity of freshly isolated healthy donor NK cells. Cells were lysed by NK cells in a dose dependent manner. MG‑63 cells exhibited less susceptibility to NK cells than HOS and U2OS cells at all cell ratios. The specific mechanism underlying the effects of NK cells on osteosarcoma cells was determined by antibody blockage experiments. The results revealed that granzyme B was the key factor in the NK cell‑induced cytotoxicity of human osteosarcoma cells. To the best of our knowledge, the present study is the first to investigate the expression of PD‑L1 in MG‑63, HOS and U2OS cells. The relative expression of the PD‑L1 gene and protein in MG‑63 cell was greater than HOS and U2OS cells. The specific lysis of human osteosarcoma cells induced by NK cells was enhanced when PD‑L1/PD‑1 was blocked by the PD‑L1 antibody. The present study proposed that the PD‑L1/PD‑1 axis serves an important role in NK cell‑induced cytotoxicity in osteosarcoma via granzyme B secretion. Our findings may contribute to the development of precise treatments for osteosarcoma based on the expression profile of PD‑L1 in patients with this disease. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::452c3072b772f7eb2f52ca5b317874d1Test https://doi.org/10.3892/or.2019.7299Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....452c3072b772f7eb2f52ca5b317874d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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