Herpes Simplex Virus Infects Skin γδ T Cells before Langerhans Cells and Impedes Migration of Infected Langerhans Cells by Inducing Apoptosis and Blocking E-Cadherin Downregulation
| العنوان: | Herpes Simplex Virus Infects Skin γδ T Cells before Langerhans Cells and Impedes Migration of Infected Langerhans Cells by Inducing Apoptosis and Blocking E-Cadherin Downregulation |
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| المؤلفون: | Ben Roediger, Cheryl A Jones, Wolfgang Weninger, Franz K. Puttur, Anthony L. Cunningham, Rose White, M. Fernandez |
| المصدر: | The Journal of Immunology. 185:477-487 |
| بيانات النشر: | The American Association of Immunologists, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Time Factors, Langerin, Herpesvirus 2, Human, Immunology, Down-Regulation, Apoptosis, Mice, Transgenic, chemical and pharmacologic phenomena, C-C chemokine receptor type 7, Herpesvirus 1, Human, medicine.disease_cause, Virus, Mice, Organ Culture Techniques, Immune system, T-Lymphocyte Subsets, medicine, Animals, Immunology and Allergy, Ear, External, integumentary system, biology, Receptors, Antigen, T-Cell, gamma-delta, hemic and immune systems, Cell Migration Inhibition, Dendritic cell, Cadherins, Virology, Mice, Inbred C57BL, Herpes simplex virus, Langerhans Cells, biology.protein, Female, Epidermis |
| الوصف: | The role individual skin dendritic cell (DC) subsets play in the immune response to HSV remains unclear. We investigated the effect of HSV on DC virus uptake, viability, and migration after cutaneous infection in vitro and in vivo. HSV increased the emigration of skin DCs from whole skin explants over 3 d postinfection (p.i.) compared with mock controls, but the kinetics of emigration was influenced by the skin DC subset. Uninfected (bystander) Langerhans cells (LCs) were the major emigrant DC subset at 24 h p.i., but thereafter, large increases in infected CD103+langerin+ dermal DC (dDC) and uninfected langerin− dDC emigration were also observed. LC infection was confirmed by the presence of HSV glycoprotein D (gD) and was associated with impaired migration from cultured skin. Langerin+ dDC also expressed HSV gD, but infection did not impede migration. We then followed the virus in live MacGreen mice in which LCs express GFP using a fluorescent HSV-1 strain by time-lapse confocal microscopy. We observed a sequential infection of epidermal cells, first in keratinocytes and epidermal γδ T cells at 6 h p.i., followed by the occurrence of HSVgD+ LCs at 24 h p.i. HSV induced CCR7 upregulation on all langerin+ DC, including infected LCs, and increased production of skin TNF-α and IL-1β. However, a large proportion of infected LCs that remained within the skin was apoptotic and failed to downregulate E-cadherin compared with bystander LCs or mock controls. Thus, HSV infection of LCs is preceded by infection of γδ T cells and delays migration. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44701e6524a2d36611dcf5a4baa5ac24Test https://doi.org/10.4049/jimmunol.0904106Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....44701e6524a2d36611dcf5a4baa5ac24 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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