Phenylpyrazole insecticides induce cytotoxicity by altering mechanisms involved in cellular energy supply in the human epithelial cell model Caco-2
| العنوان: | Phenylpyrazole insecticides induce cytotoxicity by altering mechanisms involved in cellular energy supply in the human epithelial cell model Caco-2 |
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| المؤلفون: | Jean-Luc Brunet, Alexandra Badiou, Cyril Vidau, Luc P. Belzunces |
| المساهمون: | Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU) |
| المصدر: | Toxicology in Vitro Toxicology in Vitro, Elsevier, 2009, 23, pp.589-597. ⟨10.1016/j.tiv.2009.01.017⟩ |
| بيانات النشر: | Elsevier BV, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Insecticides, CACO-2 CELL, LDH, [SDV]Life Sciences [q-bio], FIPRONIL, ENERGETIC METABILISM, Biology, Toxicology, INTESTINAL EPITHELIUM, EPITHELIUM INTESTINAL, chemistry.chemical_compound, Adenosine Triphosphate, SULFONE, SULFIDE, Electric Impedance, Humans, CYTOTOXICITY, Lactic Acid, Viability assay, PHENYLPYRAZOLE, Cytotoxicity, TEER, Barrier function, Fipronil, Cell Proliferation, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, TRANSEPITHELIAL ELECTRIC RESISTANCE, LACTATE DEHYDROGENASE, HUMAN, Epithelial Cells, CELLULE CACO-2, General Medicine, Metabolism, ETHIPROL, Intestinal epithelium, Mitochondria, ATP, METABOLITE, INTESTIN, Biochemistry, chemistry, INSECTICIDE, Caco-2, CYTOTOXICITE, Pyrazoles, Caco-2 Cells, Energy Metabolism, Xenobiotic |
| الوصف: | Phenylpyrazoles are relatively new insecticides designed to manage problematic insect resistance and public health hazards encountered with older pesticide families. In vitro cytotoxicity induced by the phenylpyrazole insecticides, Ethiprol and Fipronil, and Fipronil metabolites, sulfone and sulfide, was studied in Caco-2 cells. This cellular model was chosen because it made possible to mimic the primary site of oral exposure to xenobiotics, the intestinal epithelium. Assessment of the barrier function of Caco-2 epithelium was assessed by TEER measurement and showed a major loss of barrier integrity after exposure to Fipronil and its metabolites, but not to Ethiprol. The disruption of the epithelial barrier was attributed to severe ATP depletion independent of cell viability, as revealed by LDH release. The origin of energetic metabolism failure was investigated and revealed a transient enhancement of tetrazolium salt reduction and an increase in lactate production by Caco-2 cells, suggesting an increase in glucose metabolism by pesticides. Cellular symptoms observed in these experiments lead us to hypothesize that phenylpyrazole insecticides interacted with mitochondria |
| تدمد: | 0887-2333 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::445b589cb2fd8177907a105cc5c96666Test https://doi.org/10.1016/j.tiv.2009.01.017Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....445b589cb2fd8177907a105cc5c96666 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 08872333 |
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