Chronic Exposure to β-Alanine Generates Oxidative Stress and Alters Energy Metabolism in Cerebral Cortex and Cerebellum of Wistar Rats
| العنوان: | Chronic Exposure to β-Alanine Generates Oxidative Stress and Alters Energy Metabolism in Cerebral Cortex and Cerebellum of Wistar Rats |
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| المؤلفون: | Carlos Severo Dutra-Filho, Denise Bertin Rojas, Moacir Wajner, Gabriel H. Schirmbeck, Clovis Milton Duval Wannmacher, Cláudia Funchal, Rodrigo Binkowski de Andrade, Ângela Zanatta, Tanise Gemelli |
| المصدر: | Molecular Neurobiology. 55:5101-5110 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Taurine, Neuroscience (miscellaneous), medicine.disease_cause, Superoxide dismutase, 03 medical and health sciences, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Cerebellum, Internal medicine, Lactate dehydrogenase, medicine, Animals, Rats, Wistar, Glyceraldehyde 3-phosphate dehydrogenase, Cerebral Cortex, chemistry.chemical_classification, Reactive oxygen species, biology, Glutathione peroxidase, Oxidative Stress, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Electron Transport Chain Complex Proteins, Neurology, chemistry, Cerebral cortex, beta-Alanine, biology.protein, Energy Metabolism, Oxidative stress |
| الوصف: | β-Alanine occurs naturally in the human central nervous system and performs different functions. It can act as either a neurotransmitter or a neuromodulator, depletion of taurine levels and competitive antagonist of γ-aminobutyric acid (GABA). The β-amino acid accumulation exerts an important biological function as delay in brain development, oxidative stress and disturbances in energy metabolism, characterized as an inborn error of metabolism classified as β-alaninemia. We evaluated the effects of the chronic administration of β-alanine on some parameters of oxidative stress and enzymes of energy metabolism in cerebral cortex and cerebellum of 21-day-old Wistar rats. The animals received peritoneal injections of β-alanine (300 mg/kg of body weight), and the controls received the same volume (10 μl/g of body weight) of saline solution (NaCl 0.9%), twice a day at 12-h interval, from the 7th to the 21st postpartum day. We observed that β-amino acid was able to increase the levels of reactive oxygen species (ROS) in the two tissues; however, only in cerebral cortex total content of sulfhydryl was increased. ROS are possibly acting on antioxidant enzymes glutathione peroxidase (GPx) (cerebral cortex and cerebellum) and superoxide dismutase (SOD) (cerebellum) inhibiting their activities. We also evaluated the activities of enzymes of the phosphoryl transfer network, where we observed an increase in hexokinase and cytosolic creatine kinase (Cy-CK) activities; however, it decreased glyceraldehyde 3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PK) and lactate dehydrogenase (LDH) activities, in both tissues. Besides, the β-alanine administration increased the activities of complex II, complex IV and succinate dehydrogenase (SDH). Those results suggest that the chronic administration of β-alanine causes cellular oxidative damage, significantly changing the energy metabolism. |
| تدمد: | 1559-1182 0893-7648 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42cda2d7c0ee4143aa7f8aad847f4693Test https://doi.org/10.1007/s12035-017-0711-3Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....42cda2d7c0ee4143aa7f8aad847f4693 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15591182 08937648 |
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