Expression of carnitine palmitoyl-CoA transferase-1B is influenced by a cis-acting eQTL in two chicken lines selected for high and low body weight
| العنوان: | Expression of carnitine palmitoyl-CoA transferase-1B is influenced by a cis-acting eQTL in two chicken lines selected for high and low body weight |
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| المؤلفون: | Juleen R. Zierath, Paul B. Siegel, D. Michael Denbow, Leif Andersson, Finn Hallböök, Sojeong Ka, Svante Pääbo, Mohammad Harun-Or-Rashid, Frank W. Albert, Ellen Markljung, Henrik Ring, Pablo M. Garcia-Roves, Per Wahlberg |
| المصدر: | Physiological Genomics |
| بيانات النشر: | American Physiological Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Genotype, Physiology, Quantitative Trait Loci, Hypothalamus, Mitochondrion, Biology, Synteny, Chromosomes, Gene Expression Regulation, Enzymologic, Evolution, Molecular, Mitochondrial Proteins, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Genetics, medicine, Animals, Humans, Transferase, RNA, Messenger, Carnitine, Beta oxidation, Crosses, Genetic, Phylogeny, 030304 developmental biology, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, Polymorphism, Genetic, Base Sequence, Carnitine O-Palmitoyltransferase, Muscles, Body Weight, Chromosome Mapping, Fatty acid, Lipid metabolism, Palmitoyl-CoA, Malonyl-CoA, Biochemistry, chemistry, Organ Specificity, Multigene Family, Female, Chickens, 030217 neurology & neurosurgery, medicine.drug |
| الوصف: | Carnitine palmitoyl-CoA transferase-1B is a mitochondrial enzyme in the fatty acid oxidation pathway. In a previous study, CPT1B was identified as differentially expressed in the hypothalamus of two lines of chickens established by long-term selection for high (HWS) or low (LWS) body weight. Mammals have three paralogs ( CPT1a, b and c) while nonmammalian vertebrates only have two ( CPT1A, B). CPT1A is expressed in liver and CPT1B in muscle. CPT1c is expressed in hypothalamus, where it regulates feeding and energy expenditure. We identified an intronic length polymorphism, fixed for different alleles in the two populations, and mapped the hitherto missing CPT1B locus in the chicken genome assembly, to the distal tip of chromosome 1p. Based on molecular phylogeny and gene synteny we suggest that chicken CPT1B is pro-orthologous of the mammalian CPT1c. Chicken CPT1B was differentially expressed in both muscle and hypothalamus but in opposite directions: higher levels in hypothalamus but lower levels in muscle in the HWS than in the LWS line. Using an advanced intercross population of the lines, we found CPT1B expression to be influenced by a cis-acting expression quantitative trait locus in muscle. The increased expression in hypothalamus and reduced expression in muscle is consistent with an increased food intake in the HWS line and at the same time reduced fatty acid oxidation in muscle yielding a net accumulation of energy intake and storage. The altered expression of CPT1B in hypothalamus and peripheral tissue is likely to be a mechanism contributing to the remarkable difference between lines. |
| تدمد: | 1531-2267 1094-8341 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::420fbea1578bb1d580eac4e6d9b06421Test https://doi.org/10.1152/physiolgenomics.00078.2012Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....420fbea1578bb1d580eac4e6d9b06421 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15312267 10948341 |
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