Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo
| العنوان: | Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo |
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| المؤلفون: | Yu-An Lu, Min-Cheol Kang, Yu-Lin Dai, Jiang-Bo Yu, You-Jin Jeon, Yunfei Jiang |
| المصدر: | Toxicology Reports, Vol 8, Iss, Pp 349-358 (2021) Toxicology Reports |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DCFH-DA, 2, 7-dichlorofluorescein diacetate, Health, Toxicology and Mutagenesis, Fucoxanthin, Inflammation, SE, Standard error, COX, Cyclooxygenase, 010501 environmental sciences, Toxicology, NO, Nitric oxide, H-PM, Culture medium of PM-induced keratinocytes, 01 natural sciences, Nitric oxide, DMEM, Dulbecco's Modified Eagle's Medium, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, RA1190-1270, medicine, TNF-α, Tumor necrosis factor-α, IL, Interleukin, 0105 earth and related environmental sciences, chemistry.chemical_classification, Reactive oxygen species, Sargassum fusiformis, biology, FxRF, Fucoxanthin-rich fraction, Interleukin, Regular Article, PM, Particulate matter, PI, Propidium iodide, Molecular biology, Nitric oxide synthase, HaCaT, PGE, Prostaglandin E, chemistry, Toxicology. Poisons, biology.protein, Tumor necrosis factor alpha, Anti-inflammatory response, medicine.symptom, Fx, Fucoxanthin, iNOS, Inducible nitric oxide synthases, Particulate matter, 030217 neurology & neurosurgery |
| الوصف: | Particulate matter (PM) contributes to air pollution and primarily originates from unregulated industrial emissions and seasonal natural dust emissions. Fucoxanthin (Fx) is a marine natural pigment from brown macroalgae that has been shown to have various beneficial effects on health. However, the effects of Fx on PM-induced toxicities in cells and animals have not been assessed. In this study, we investigated the anti-inflammatory potential of the Fx-rich fraction (FxRF) of Sargassum fusiformis against PM-mediated inflammatory responses. The FxRF composition was analyzed by rapid-resolution liquid chromatography mass spectrometry. Fx and other main pigments were identified. FxRF attenuated the production of inflammatory components, including prostaglandin E2 (PGE2), cyclooxygenase-2, interleukin (IL)-1β, and IL-6 from PM-exposed HaCaT keratinocytes. PM exposure also reduced the levels of nitric oxide (NO), tumor necrosis factor-α, inducible nitric oxide synthase (iNOS), and PGE2 in PM-exposed RAW264.7 macrophages. Additionally, the culture medium from PM-exposed HaCaT cells induced upregulation of NO, iNOS, PGE2, and pro-inflammatory cytokines in RAW264.7 macrophages. FxRF also significantly decreased the expression levels of factors involved in inflammatory responses, such as NO, reactive oxygen species, and cell death, in PM-exposed zebrafish embryos. These results demonstrated the potential protective effects of FxRF against PM-induced inflammation both in vitro and in a zebrafish model. |
| اللغة: | English |
| تدمد: | 2214-7500 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3eb715c1d24f511ba48f5dae8bec71c6Test http://www.sciencedirect.com/science/article/pii/S2214750021000226Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3eb715c1d24f511ba48f5dae8bec71c6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22147500 |
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