Endosomal recycling tubule scission and integrin recycling involve the membrane curvature-supporting protein LITAF
| العنوان: | Endosomal recycling tubule scission and integrin recycling involve the membrane curvature-supporting protein LITAF |
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| المؤلفون: | Martin Lowe, Wenxia Qin, Rebecca Yarwood, Philip G. Woodman, Lydia Wunderley, Ling Zhang |
| المصدر: | Journal of Cell Science article-version (VoR) Version of Record |
| بيانات النشر: | The Company of Biologists, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lipopolysaccharides, Integrins, ESCRT machinery, Endosome, Integrin, Cell, Endosomes, LITAF, SNX, 03 medical and health sciences, 0302 clinical medicine, Charcot-Marie-Tooth Disease, medicine, Humans, 030304 developmental biology, 0303 health sciences, biology, Membrane Proteins, Nuclear Proteins, Cell migration, Cell Biology, Cell biology, Tubule, medicine.anatomical_structure, Membrane, Membrane protein, Membrane curvature, Rab11, Charcot Marie Tooth, biology.protein, Recycling endosome, EHD, 030217 neurology & neurosurgery, Transcription Factors, Research Article |
| الوصف: | Recycling to the cell surface requires the scission of tubular membrane intermediates emanating from endosomes. Here, we identify the monotopic membrane protein LPS-induced TNF-activating factor (LITAF) and the related protein cell death involved p53 target 1 (CDIP1) as novel membrane curvature proteins that contribute to recycling tubule scission. Recombinant LITAF supports high membrane curvature, shown by its ability to reduce proteoliposome size. The membrane domains of LITAF and CDIP1 partition strongly into ∼50 nm diameter tubules labelled with the recycling markers Pacsin2, ARF6 and SNX1, and the recycling cargoes MHC class I and CD59. Partitioning of LITAF into tubules is impaired by mutations linked to Charcot Marie Tooth disease type 1C. Meanwhile, co-depletion of LITAF and CDIP1 results in the expansion of tubular recycling compartments and stabilised Rab11 tubules, pointing to a function for LITAF and CDIP1 in membrane scission. Consistent with this, co-depletion of LITAF and CDIP1 impairs integrin recycling and cell migration. Summary: LITAF is an endosomal monotopic membrane protein that is mutated in Charcot Marie Tooth disease type 1C. We show that LITAF is important for endosomal recycling through supporting the scission of recycling tubules. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e4fe6a65faabce1cbcba1f86b8facefTest https://doi.org/10.1242/jcs.258549Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3e4fe6a65faabce1cbcba1f86b8facef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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