A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
| العنوان: | A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
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| المؤلفون: | Jean-Christophe Avice, Marie-Françoise Niogret, Alain Bouchereau, Philippe Etienne, Marine Poret, Françoise Le Cahérec, Alexandra Girondé, Jacques Trouverie, Laurent Leport |
| المساهمون: | Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Rennes 1 (UR1), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Amélioration des Plantes et Biotechnologies Végétales (APBV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| المصدر: | Plants, Vol 5, Iss 1, p 1 (2015) Plants Plants, MDPI, 2016, 5 (1), pp.1. ⟨10.3390/plants5010001⟩ Plants; Volume 5; Issue 1; Pages: 1 Plants, 2016, 5 (1), pp.1. ⟨10.3390/plants5010001⟩ |
| بيانات النشر: | MDPI AG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0106 biological sciences, Proteases, proteolysis, medicine.medical_treatment, Proteolysis, Plant Science, Vacuole, Biology, Protein degradation, Brassica napus, N remobilization, genotypic variability, acidic proteases, 01 natural sciences, Article, Serine, 03 medical and health sciences, Abscission, lcsh:Botany, Botany, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant breeding, ComputingMilieux_MISCELLANEOUS, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Protease, Ecology, medicine.diagnostic_test, food and beverages, lcsh:QK1-989, Biochemistry, 010606 plant biology & botany |
| الوصف: | Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2223-7747 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c43b1993cb97d1197f5bac4a316a2fcTest http://www.mdpi.com/2223-7747/5/1/1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3c43b1993cb97d1197f5bac4a316a2fc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22237747 |
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