Phenotypic and functional characterization of natural killer cells in rheumatoid arthritis-regulation with interleukin-15
| العنوان: | Phenotypic and functional characterization of natural killer cells in rheumatoid arthritis-regulation with interleukin-15 |
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| المؤلفون: | Chien-Ya Hsu, Hsiu-Shan Hsiao, Ming-Ling Kuo, Pei-Tzu Lee, Syh-Jae Lin, Ji-Yih Chen |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, CD3, medicine.medical_treatment, lcsh:Medicine, Inflammation, Article, Arthritis, Rheumatoid, 03 medical and health sciences, Young Adult, 0302 clinical medicine, medicine, Humans, L-Selectin, Rheumatoid arthritis, lcsh:Science, Aged, Autoimmune disease, Aged, 80 and over, Interleukin-15, Multidisciplinary, biology, business.industry, Natural Cytotoxicity Triggering Receptor 1, Interleukins, lcsh:R, Interleukin, Receptors, KIR3DL1, Middle Aged, medicine.disease, Granzyme B, Killer Cells, Natural, 030104 developmental biology, Cytokine, Perforin, Interleukin 15, Receptors, KIR2DL3, Case-Control Studies, Immunology, biology.protein, lcsh:Q, Female, medicine.symptom, business, Immunologic Memory, 030215 immunology |
| الوصف: | Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation and joint destruction. Previous studies have shown that natural killer (NK) cells may play an important role in the pathogenesis of RA. Interleukin (IL)-15, a pro-inflammatory cytokine which induces proliferation and differentiation of NK cells, is overexpressed in RA. In this present study, we examine various NKRs and adhesion molecule expression on NK cells from RA patients and their response to IL-15 stimulation. We also sought to study cytokine-induced memory-like (CIML) NK cells in RA patients. We established that 1. RA patients had higher NK cell percentages in peripheral blood and their serum IL-15 levels were higher compared to healthy volunteers; 2. NK cells from RA patients showed lower NKp46 expression and an impaired CD69 response to IL-15; 3. NK cells from RA patients showed higher CD158b and CD158e expression but lower CD62L expression; 4. exogenous IL-15 up-regulated CD69, CD158b, CD158e but down-regulated NKp46 and CD62L expression in RA; 5. As to CIML NK cells, restimulation - induced NK cytotoxicity and IFN-γ production was impaired in RA patients, 6. Reduced NKp46, perforin, and granzyme B expression on NK cells was found in RA patients with bone deformity and erosion, 7. RA disease activity (DAS28) showed inverse correlation with the percentages of CD56+CD3− NK cells, and NKp46 and perforin expression on NK cells, respectively. Taken together, our study demonstrated differential expression of various NK receptors in RA patients. NKp46, CD158e, and perforin expression on NK cells may serve as markers of RA severity. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b7e413279cbd963749f8f8fd19f6f57Test https://pubmed.ncbi.nlm.nih.gov/32246007Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3b7e413279cbd963749f8f8fd19f6f57 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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