Cytokine and Growth Factor Expression by HTLV-1 Lck-taxTransgenic Cells in SCID Mice
| العنوان: | Cytokine and Growth Factor Expression by HTLV-1 Lck-taxTransgenic Cells in SCID Mice |
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| المؤلفون: | William W. Hall, Hideki Hasegawa, Karen M. Watters, Hirofumi Sawa, Jonathan L.E. Dean, Noreen Sheehy |
| المصدر: | AIDS Research and Human Retroviruses. 26:593-603 |
| بيانات النشر: | Mary Ann Liebert Inc, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Genetically modified mouse, Interleukin 2, Transgene, medicine.medical_treatment, Immunology, Becaplermin, Vascular Cell Adhesion Molecule-1, Mice, Transgenic, Mice, SCID, Biology, Mice, Interferon, Virology, medicine, Animals, Humans, Interleukin 3, Platelet-Derived Growth Factor, Human T-lymphotropic virus 1, Tumor Necrosis Factor-alpha, Growth factor, Gene Products, tax, Proto-Oncogene Proteins c-sis, Intercellular Adhesion Molecule-1, Disease Models, Animal, Infectious Diseases, Cytokine, Gene Expression Regulation, Interleukin 15, Cytokines, Intercellular Signaling Peptides and Proteins, medicine.drug |
| الوصف: | The Tax protein encoded by HTLV-1 plays a key role in the development of ATL in infected individuals. Our previous studies showed that tax transgenic mice develop disease that is almost identical to human ATL, with widespread organ invasion by lymphomatous cells and the development of leukemia. The same pathology develops rapidly in SCID mice engrafted with cells from the transgenic animals. In the present study, we used this SCID model to analyze the expression levels of several cytokines, growth factors, and adhesion molecules to determine their possible involvement in the development of disease. We showed that Tax expression was undetectable at the protein level in the tax-transformed cells used to inoculate the SCID mice and that these cells displayed constitutive NF-kappaB and Akt activity. We demonstrated significant differences in the levels of circulating PDGF-BB, TNF-alpha, sICAM-1, and sVCAM-1 in inoculated animals. Cell-surface staining of the tax transgenic cells showed that they do not express receptors for any of the upregulated growth factors. Significant differences were not found in the secreted levels of bFGF, MMP9, VEGF, or E-selectin, whereas IL-2, IL-15, IL-6, IL-1beta, and IFN-gamma expression was undetectable. Even though the number of factors analyzed is limited, our study identified TNF-alpha, PDGF-BB, and the adhesion molecules sICAM-1 and sVCAM-1 as factors that may contribute to the high levels of organ infiltration by leukemic cells in this tax transgenic SCID model. |
| تدمد: | 1931-8405 0889-2229 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3446e8d383c65fae4b8b47ddd4c87fdcTest https://doi.org/10.1089/aid.2009.0212Test |
| رقم الانضمام: | edsair.doi.dedup.....3446e8d383c65fae4b8b47ddd4c87fdc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19318405 08892229 |
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