ASK 1 inhibitor treatment suppresses p38/JNK signalling with reduced kidney inflammation and fibrosis in rat crescentic glomerulonephritis
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| المؤلفون: | John T. Liles, Frank Y. Ma, Liv Amos, David J. Nikolic-Paterson, Greg H. Tesch, David G. Breckenridge, Yingjie Han |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, T-Lymphocytes, Kidney Glomerulus, urologic and male genital diseases, Rats, Inbred WKY, p38 Mitogen-Activated Protein Kinases, Rats, Sprague-Dawley, Glomerulonephritis, Cell Movement, Fibrosis, Medicine, Rapidly progressive glomerulonephritis, ASK1, Phosphorylation, Kinase, 3. Good health, Proteinuria, Molecular Medicine, Female, Original Article, medicine.symptom, Signal Transduction, medicine.medical_specialty, p38 mitogen-activated protein kinases, Inflammation, macrophage, p38 MAPK, MAP Kinase Kinase Kinase 5, Nephrotoxicity, 03 medical and health sciences, Internal medicine, Renal fibrosis, Animals, Humans, Protein Kinase Inhibitors, thrombosis, business.industry, Macrophages, JNK Mitogen-Activated Protein Kinases, Original Articles, Cell Biology, Macrophage Activation, medicine.disease, Rats, Disease Models, Animal, 030104 developmental biology, Endocrinology, Gene Expression Regulation, inflammation, JNK, business |
| الوصف: | Activation of p38 mitogen‐activated protein kinase (MAPK) and c‐Jun amino terminal kinase (JNK) is prominent in human crescentic glomerulonephritis. p38 and JNK inhibitors suppress crescentic disease in animal models; however, the upstream mechanisms inducing activation of these kinases in crescentic glomerulonephritis are unknown. We investigated the hypothesis that apoptosis signal‐regulating kinase 1 (ASK1/MAP3K5) promote p38/JNK activation and renal injury in models of nephrotoxic serum nephritis (NTN); acute glomerular injury in SD rats, and crescentic disease in WKY rats. Treatment with the selective ASK1 inhibitor, GS‐444217 or vehicle began 1 hour before nephrotoxic serum injection and continued until animals were killed on day 1 (SD rats) or 14 (WKY rats). NTN resulted in phosphorylation (activation) of p38 and c‐Jun in both models which was substantially reduced by ASK1 inhibitor treatment. In SD rats, GS‐444217 prevented proteinuria and glomerular thrombosis with suppression of macrophage activation on day 1 NTN. In WKY rats, GS‐444217 reduced crescent formation, prevented renal impairment and reduced proteinuria on day 14 NTN. Macrophage activation, T‐cell infiltration and renal fibrosis were also reduced by GS‐444217. In conclusion, GS‐444217 treatment inhibited p38/JNK activation and development of renal injury in rat NTN. ASK1 inhibitors may have therapeutic potential in rapidly progressive glomerulonephritis. |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::339b4885367b1c644a0c188e5c9be022Test https://doi.org/10.1111/jcmm.13705Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....339b4885367b1c644a0c188e5c9be022 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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