Technology for obtaining an ultrathin posterior lamellar corneal graft at the Eye Tissue Bank
| العنوان: | Technology for obtaining an ultrathin posterior lamellar corneal graft at the Eye Tissue Bank |
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| المؤلفون: | A. K. Ahmedov, T. Z. Kerimov, Kh. D. Tonaeva, B. E. Malygin, S. A. Borzenok |
| المصدر: | Vestnik Transplantologii i Iskusstvennyh Organov, Vol 22, Iss 3, Pp 167-173 (2020) |
| بيانات النشر: | V.I. Shimakov Federal Research Center of Transplantology and Artificial Organs, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Corneal endothelium, Materials science, RD1-811, genetic structures, Scanning electron microscope, keratoplasty, Immunofluorescent microscopy, 03 medical and health sciences, 0302 clinical medicine, Optical coherence tomography, Microkeratome, Cornea, Microscopy, medicine, Immunology and Allergy, Transplantation, medicine.diagnostic_test, Eye bank, ultrathin lamellar corneal graft, preservation solution, eye diseases, eye bank, medicine.anatomical_structure, 030221 ophthalmology & optometry, Surgery, sense organs, 030217 neurology & neurosurgery, Biomedical engineering |
| الوصف: | Objective: to develop technologies for preoperative preparation of the posterior lamellar corneal graft based on our own formulation of the preservation medium for optimal dehydration of the donor cornea and a technique for cutting out an ultrathin flap using an optimized method at the Eye Tissue Bank. Materials methods. In a series of experimental studies, we obtained data on the hydration level of cadaveric donor corneas that were preserved in various solutions at different observation periods. Using 16 corneas, analytical weighing and pachymetry were performed via optical coherence tomography in the experimental (n = 8) and control (n = 8) groups. Morphological and functional characteristics of the corneal endothelium were then assessed. At the next stage of work, ultrathin grafts were formed from 16 corneas after hypothermic preservation in the experimental (n = 8) and control (n = 8) solutions by single-pass microkeratome, followed by microscopy of the samples using a scanning electron microscope. Results. After the first days of preservation in the proposed solution, there was dehydration of 9% cornea in the experimental group in comparison with the samples of the control group. After 4 days of preservation, there was no reliable difference found between the groups (p > 0.05) in the study of the endothelial cell viability of ultra-thin corneal grafts by immunofluorescent microscopy using the «Live and dead» marker. Scanning electron microscopy revealed that corneal stromal collagen fibers, preserved in the proposed medium, retained their integrity. Conclusion. The proposed technology can be recommended for use at eye banks for formation of an ultra-thin corneal graft at the preoperative stage. |
| تدمد: | 2412-6160 1995-1191 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::326baf4a2bb27f1bce9fc94beb5fbe8eTest https://doi.org/10.15825/1995-1191-2020-3-167-173Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....326baf4a2bb27f1bce9fc94beb5fbe8e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24126160 19951191 |
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