In Situ Hybridization of PRRSV-1 Combined with Digital Image Analysis in Lung Tissues of Pigs Challenged with PRRSV-1
| العنوان: | In Situ Hybridization of PRRSV-1 Combined with Digital Image Analysis in Lung Tissues of Pigs Challenged with PRRSV-1 |
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| المؤلفون: | Poul H. Ratkhjen, Till Rümenapf, Oliver Duran, Attila Marcell Szász, Gyula Balka, Andrea Ladinig, Christian Kraft, Balazs Acs, Márton Papp, Dávid G. Horváth, Lilla Dénes |
| المصدر: | Veterinary Sciences; Volume 8; Issue 10; Pages: 235 Veterinary Sciences, Vol 8, Iss 235, p 235 (2021) Veterinary Sciences |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lung, General Veterinary, biology, Veterinary medicine, animal diseases, RNA, qRT-PCR, In situ hybridization, porcine reproductive and respiratory syndrome virus, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Virology, Article, Virus, Reverse transcription polymerase chain reaction, medicine.anatomical_structure, Real-time polymerase chain reaction, QuPath, SF600-1100, medicine, in situ hybridization, RNAscope, Respiratory system |
| الوصف: | Betaarterivirus suid 1 and 2 are the causative agents of porcine reproductive and respiratory syndrome (PRRS), which is one of the most significant diseases of the swine industry, causing significant economic losses in the main pig producing countries. Here, we report the development of a novel, RNA-based in situ hybridization technique (RNAscope) to detect PRRS virus (PRRSV) RNA in lung tissues of experimentally infected animals. The technique was applied to lung tissues of 20 piglets, which had been inoculated with a wild-type, highly pathogenic PRRSV-1 strain. To determine the RNAscope’s applicability as a semi-quantitative method, we analysed the association between the proportion of the virus-infected cells measured with an image analysis software (QuPath) and the outcome of the real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) tests performed in parallel. The results of the quantitative approach of these two molecular biological methods show significant association (pseudo R2 = 0.3894, p = 0.004). This is the first time RNAscope assay has been implemented for the detection of PRRSV-1 in experimental animals. |
| وصف الملف: | application/pdf |
| تدمد: | 2306-7381 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::325162e2f36f0424f853fac5dd887af8Test https://doi.org/10.3390/vetsci8100235Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....325162e2f36f0424f853fac5dd887af8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23067381 |
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