SOCS3 Is Upregulated and Targeted by miR30a-5p in Allergic Rhinitis
| العنوان: | SOCS3 Is Upregulated and Targeted by miR30a-5p in Allergic Rhinitis |
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| المؤلفون: | Hong Chao Yao, Xin Wang, Wei Wang, Chun Yuan Zhao |
| المصدر: | International Archives of Allergy and Immunology. 175:209-219 |
| بيانات النشر: | S. Karger AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Immunology, Biology, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Animals, Humans, Immunology and Allergy, T-helper cell differentiation, SOCS3, Mice, Inbred BALB C, Suppressor of cytokine signaling 1, digestive, oral, and skin physiology, General Medicine, Middle Aged, Rhinitis, Allergic, Up-Regulation, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, Suppressor of Cytokine Signaling 3 Protein, Female, 030215 immunology |
| الوصف: | Background: Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 play important roles in T helper cell differentiation, which is involved with the pathologic mechanisms of allergic rhinitis (AR). The aim of this study was to evaluate the expression of SOCS1 and SOCS3 in AR and find their regulatory microRNAs (miRNAs) to provide a basis for the treatment of AR. Methods: The expression of SOCS1 and SOCS3 were analyzed by real-time PCR, immunohistochemistry, and Western blot. The correlative regulatory miRNAs were detected by real-time PCR. Luciferase assays and AR mouse model experiments were applied to identify correlative miRNAs that target SOCS3. Results: SOCS1 and SOCS3 mRNA were upregulated in the nasal mucosa and peripheral blood mononuclear cells of AR compared with controls. The expression of SOCS3 protein was significantly increased in the nasal mucosa of AR. The immunohistochemical staining results showed that SOCS3 was similarly localized in the superficial epithelium, submucosal glands, and vascular endothelium in the nasal mucosa of AR subjects and controls. However, SOCS3 protein was especially localized in the inflammatory cells, such as eosinophils, monocytes, and lymphocytes. Conclusions: SOCS3 was targeted by miR30a- 5p in AR. Further study should be performed to identify the regulatory effect of miR30a-5p in AR, which may provide insights into a new therapeutic strategy. |
| تدمد: | 1423-0097 1018-2438 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::319f7dd84fb3a2ffcde96b3e7ceb3746Test https://doi.org/10.1159/000486857Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....319f7dd84fb3a2ffcde96b3e7ceb3746 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230097 10182438 |
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