De-osteogenic-differentiated mesenchymal stem cells accelerate fracture healing by mir-92b
| العنوان: | De-osteogenic-differentiated mesenchymal stem cells accelerate fracture healing by mir-92b |
|---|---|
| المؤلفون: | Ying Li, Weiping Lin, Yamei Liu, Xiaohua Jiang, Yonghui Hou, Yuxin Sun, Chen Chen, Gang Li, Liangliang Xu |
| المصدر: | Journal of Orthopaedic Translation, Vol 27, Iss, Pp 25-32 (2021) Journal of Orthopaedic Translation |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | lcsh:Diseases of the musculoskeletal system, Mesenchymal stem cell, De-Os-MSCs, Bone healing, Biology, Regenerative medicine, In vitro, Fracture, Tissue engineering, Mir-92b, In vivo, microRNA, Cancer research, Mesenchymal stem cells, Orthopedics and Sports Medicine, Original Article, lcsh:RC925-935, Bone regeneration |
| الوصف: | Background Mesenchymal stem cells (MSCs) are promising targets for therapeutic use in regenerative medicine and tissue engineering. In the previous study, we have found that MSCs could be reverted to a primitive stem cell population after in vitro induction of osteogenic and de-osteogenic differentiation (de-osteogenic differentiated MSCs, De-Os-MSCs). De-Os-MSCs showed improved cell survival and osteogenic potential. However, the underlying mechanism and its potential effect on fracture healing has not been explored. Methods MSCs were isolated from the rat bone marrow. MicroRNAs were cloned into lentiviral vectors and transduced into MSCs to observe the effects on osteogenesis. The expression levels of marker genes were evaluated by quantitative RT-PCR. Ectopic bone formation model was used to evaluate the bone regeneration ability of mir-92b transduced MSCs in vivo. An open femur fracture model was established, and MSCs or De-Os-MSCs were administrated to the fracture sites. Histological, biomechanical and microCT analysis were used to evaluate the quality of bone. Results In the present study, we found that mir-92b was significantly increased in the secretions of De-Os-MSCs. And mir-92b could promote the osteogenic differentiation potential of MSCs by activating pERK and JNK signaling pathways. The ectopic bone formation assay showed that MSCs overexpressing mir-92b formed more bone like tissues in vivo. Most importantly, we found local administration of De-Os-MSCs could accelerate fracture healing using an open femur fracture model in rats. The quality of bone property was much better as shown by microCT and biomechanical testing. Conclusion Taken together, our study demonstrated that mir-92b promoted osteogenesis of MSCs, which was partially accounted for the enhanced osteogenic differentiation potential of De-Os-MSCs. And De-Os-MSCs had shown better regenerative capacity in accelerating fracture healing when they were locally given. The translational potential of this article De-Os-MSCs could be used to accelerate fracture healing, and reduce the occurrence of delayed unions and non-unions. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30ddfd0b16eb4cf9bea174938d6ff759Test http://www.sciencedirect.com/science/article/pii/S2214031X20301224Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30ddfd0b16eb4cf9bea174938d6ff759 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |