Determination of plasma non-esterified fatty acids in herbivores and man : a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods
| العنوان: | Determination of plasma non-esterified fatty acids in herbivores and man : a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods |
|---|---|
| المؤلفون: | Dominique Bauchart, Jacques Barnouin, Jeannette Flechet, Françoise Duboisset, Yves Chilliard, Jean-Paul Chacornac |
| المساهمون: | Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Unité de Recherche d'Écopathologie, Revues Inra, Import |
| المصدر: | Reproduction Nutrition Développement Reproduction Nutrition Développement, 1984, 24 (4), pp.469-482 Reproduction Nutrition Development Reproduction Nutrition Development, EDP Sciences, 1984, 24 (4), pp.469-482 Reproduction Nutrition Development 4 (24), 469-482. (1984) |
| بيانات النشر: | HAL CCSD, 1984. |
| سنة النشر: | 1984 |
| مصطلحات موضوعية: | Embryology, Chemical Phenomena, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), Ether, Fatty Acids, Nonesterified, [SHS]Humanities and Social Sciences, 03 medical and health sciences, chemistry.chemical_compound, NEFA, [SDV.BDD] Life Sciences [q-bio]/Development Biology, Methods, Animals, Humans, [INFO]Computer Science [cs], Horses, Bovine serum albumin, Colorimetry, [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Phenol red, chemistry.chemical_classification, 0303 health sciences, Chromatography, Autoanalysis, biology, Goats, Extraction (chemistry), 0402 animal and dairy science, Fatty acid, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 04 agricultural and veterinary sciences, 040201 dairy & animal science, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Chemistry, Reproductive Medicine, chemistry, biology.protein, Animal Science and Zoology, Titration, Cattle, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Developmental Biology, Food Science |
| الوصف: | National audience; Non-esterified fatty acid (NEFA) contents were determined (1) in cow, goat, mare and human plasmas or sera and (2) in bovine serum albumin solutions by thin-layer and gas-liquid chromatographic methods which were compared with 6 chemical methods of determination and 2 enzymatic methods (manual and automatic). The chemical methods combined two extraction techniques (isopropanol-heptane and silicic acid-diisopropyl ether) and three determination methods (titrimetry and colorimetry using either phenol red or copper soaps). Correlations between the nine various methods were high, but in some cases regression lines were different from the bissector and species-dependent. The putative occurrence of interfering compounds or the possible effect of between-species differences in NEFA composition have been discussed. The choice of a fast method depends primarily on practical considerations, but the need to standardize each method for a given species has been stressed. Automatic enzymatic assay seemed to be suitable for routine analyses. |
| وصف الملف: | application/pdf |
| اللغة: | French |
| تدمد: | 0926-5287 1297-9708 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30745fdcfbfb18c8ce890367dc51bb26Test https://hal.archives-ouvertes.fr/hal-00898168/file/hal-00898168.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30745fdcfbfb18c8ce890367dc51bb26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09265287 12979708 |
|---|