Rhododenol Activates Melanocytes and Induces Morphological Alteration at Sub-Cytotoxic Levels
| العنوان: | Rhododenol Activates Melanocytes and Induces Morphological Alteration at Sub-Cytotoxic Levels |
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| المؤلفون: | Minjeong Kim, Chang Seok Lee, Kyung Min Lim |
| المصدر: | International Journal of Molecular Sciences Volume 20 Issue 22 |
| بيانات النشر: | MDPI, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Leukoderma, Butanols, Cell, rhododenol, Vitiligo, Human skin, chemical and pharmacologic phenomena, Biology, Melanocyte, Models, Biological, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Mice, 0302 clinical medicine, Cell Line, Tumor, morphology, medicine, Cytotoxic T cell, leukoderma, Animals, Humans, Trypsin, Physical and Theoretical Chemistry, Cytoskeleton, Molecular Biology, Spectroscopy, Melanosome, integumentary system, Dose-Response Relationship, Drug, Monophenol Monooxygenase, Organic Chemistry, cytoskeleton, General Medicine, medicine.disease, Computer Science Applications, Cell biology, melanocytes, Cytoskeletal Proteins, 030104 developmental biology, medicine.anatomical_structure, Gene Expression Regulation, 030220 oncology & carcinogenesis, Melanoderm™ |
| الوصف: | Rhododenol (RD), a whitening cosmetic ingredient, was withdrawn from the market due to RD-induced leukoderma (RIL). While many attempts have been made to clarify the mechanism underlying RIL, RIL has not been fully understood yet. Indeed, affected subjects showed uneven skin pigmentation, but the features are different from vitiligo, a skin hypopigmentary disorder, alluding to events more complex than simple melanocyte cytotoxicity. Here, we discovered that rhododenol treatment reduced the number of melanocytes in a pigmented 3D human skin model, Melanoderm&trade confirming the melanocyte toxicity of RD. Of note, melanocytes that survived in the RD treated tissues exhibited altered morphology, such as extended dendrites and increased cell sizes. Consistently with this, sub-cytotoxic level of RD increased cell size and elongated dendrites in B16 melanoma cells. Morphological changes of B16 cells were further confirmed in the immunocytochemistry of treated cells for actin and tubulin. Even more provoking, RD up-regulated the expression of tyrosinase and TRP1 in the survived B16 cells. Evaluation of mRNA expression of cytoskeletal proteins suggests that RD altered the cytoskeletal dynamic favoring cell size expansion and melanosome maturation. Collectively, these results suggest that RD not only induces cytotoxicity in melanocytes but also can lead to a profound perturbation of melanocyte integrity even at sub-cytotoxic levels. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::301ed88a67444150bf20889465a7e179Test http://europepmc.org/articles/PMC6888388Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....301ed88a67444150bf20889465a7e179 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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