Upregulation of miR-345-5p suppresses cell growth of lung adenocarcinoma by regulating ras homolog family member A (RhoA) and Rho/Rho associated protein kinase (Rho/ROCK) pathway
| العنوان: | Upregulation of miR-345-5p suppresses cell growth of lung adenocarcinoma by regulating ras homolog family member A (RhoA) and Rho/Rho associated protein kinase (Rho/ROCK) pathway |
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| المؤلفون: | Qiao-Yun Zhou, Shu-Yu Gui, Peng Zhang, Mei Wang, Pei-Fang Wei |
| المصدر: | Chinese Medical Journal, Vol 134, Iss 21, Pp 2619-2628 (2021) Chinese Medical Journal |
| بيانات النشر: | Wolters Kluwer, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lung adenocarcinoma, RHOA, Lung Neoplasms, Adenocarcinoma of Lung, Biology, Ras homolog family member A (RhoA), MicroRNA-345-5p, Downregulation and upregulation, Cell Movement, Cell Line, Tumor, medicine, Humans, Rho-associated protein kinase, Cell Proliferation, rho-Associated Kinases, Cell growth, General Medicine, Original Articles, Rho/Rho-associated protein kinase (ROCK), medicine.disease, Up-Regulation, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell culture, Apoptosis, Cancer research, biology.protein, Adenocarcinoma, Medicine, Signal transduction, rhoA GTP-Binding Protein |
| الوصف: | Background:. Microribose nucleic acids (miRNAs) are implicated in the progression of lung adenocarcinoma. MicroRNA-345-5p (miR-345-5p) is a recently identified anti-oncogene in some human cancers, but its functional role and possible molecular mechanism in lung adenocarcinoma remain unknown. This study aimed to identify the biological function and underlying mechanism of miR-345-5p in lung adenocarcinoma cells. Methods:. In this study, lung adenocarcinoma tissues and adjacent tissues were collected in the First Affiliated Hospital of Anhui Medical University between April 2016 and February 2017. The expression of miR-345-5p and ras homolog family member A (RhoA) in lung adenocarcinoma tissues and human lung adenocarcinoma cell lines (A549, H1650, PC-9, and H441) was detected by reverse transcription quantitative polymerase chain reaction analysis. Functional assays including colony formation, flow cytometry analysis, wound healing, and transwell assays were performed to assess the proliferation, apoptosis, migration, and invasion of lung adenocarcinoma cells. In addition, RNA pulldown and luciferase reporter assays were conducted to evaluate the relationship between miR-345-5p and RhoA. Difference between the two groups was analyzed with Student's t test, while that among multiple groups was analyzed with one-way analysis of variance. Results:. MiR-345-5p expression displayed lower level in lung adenocarcinoma tissues (0.241 ± 0.095 vs.1.000 ± 0.233, t = 19.247, P < 0.001) and cell lines (F = 56.992, P < 0.001) than control tissues and cells. Functional experiments demonstrated that upregulation of miR-345-5p inhibited the malignant phenotypes of lung adenocarcinoma cells via suppressing cell proliferation, migration, invasion, and facilitating cell apoptosis. Additionally, RhoA was verified to be the downstream target of miR-345-5p. Expression of RhoA was downregulated by overexpression of miR-345-5p in PC-9 (0.321 ± 0.047 vs. 1.000 ± 0.127, t = 8.536, P < 0.001) and H1650 (0.398 ± 0.054 vs. 1.000 ± 0.156, t = 4.429, P = 0.011) cells. Rescue assays revealed that overexpression of RhoA rescued the suppressive effects of miR-345-5p upregulation on proliferation, migration, and invasion of lung adenocarcinoma cells. Further, miR-345-5p was found to regulate the Rho/Rho-associated protein kinase (ROCK) signaling pathway by downregulation of RhoA in lung adenocarcinoma cells. Conclusions:. MiR-345-5p plays a tumor suppressor role in lung adenocarcinoma cells by downregulating RhoA to inactivate the Rho/ROCK pathway. |
| اللغة: | English |
| تدمد: | 2542-5641 0366-6999 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ff652b63ad887978ae8106133427eedTest http://journals.lww.com/10.1097/CM9.0000000000001804Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ff652b63ad887978ae8106133427eed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25425641 03666999 |
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