Identification of differentially expressed genes in fibroblasts derived from patients with Dupuytren's Contracture
| العنوان: | Identification of differentially expressed genes in fibroblasts derived from patients with Dupuytren's Contracture |
|---|---|
| المؤلفون: | Garth D. Ehrlich, Latha Satish, William A. LaFramboise, Mark E. Baratz, Sandra Johnson, Sandeep Kathju, David B. O’Gorman, Benjamin Janto, Fen Z. Hu, J. Christopher Post, Bing Siang Gan |
| المصدر: | Surgery Publications BMC Medical Genomics BMC Medical Genomics, Vol 1, Iss 1, p 10 (2008) |
| بيانات النشر: | Scholarship@Western, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Candidate gene, lcsh:Internal medicine, Microarray, lcsh:QH426-470, Medical Physiology, Biology, Bioinformatics, Significance Analysis of Microarrays, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, Proteoglycan 4, Dupuytren's contracture, Gene expression, medicine, Genetics, Genetics(clinical), lcsh:RC31-1245, Gene, Genetics (clinical), 030304 developmental biology, 0303 health sciences, medicine.disease, body regions, lcsh:Genetics, 030220 oncology & carcinogenesis, Significance analysis of microarrays, Cancer research, biology.protein, Surgery, Medical Genetics, Research Article |
| الوصف: | Dupuytren's contracture (DC) is the most common inherited connective tissue disease of humans and is hypothesized to be associated with aberrant wound healing of the palmar fascia. Fibroblasts and myofibroblasts are believed to play an important role in the genesis of DC and the fibroproliferation and contraction that are hallmarks of this disease. This study compares the gene expression profiles of fibroblasts isolated from DC patients and controls in an attempt to identify key genes whose regulation might be significantly altered in fibroblasts found within the palmar fascia of Dupuytren's patients. Total RNA isolated from diseased palmar fascia (DC) and normal palmar fascia (obtained during carpal tunnel release; 6 samples per group) was subjected to quantitative analyses using two different microarray platforms (GE Code Link™ and Illumina™) to identify and validate differentially expressed genes. The data obtained was analyzed using The Significance Analysis of Microarrays (SAM) software through which we identified 69 and 40 differentially regulated gene transcripts using the CodeLink™ and Illumina™ platforms, respectively. The CodeLink™ platform identified 18 upregulated and 51 downregulated genes. Using the Illumina™ platform, 40 genes were identified as downregulated, eleven of which were identified by both platforms. Quantitative RT-PCR confirmed the downregulation of three high-interest candidate genes which are all components of the extracellular matrix: proteoglycan 4 (PRG4), fibulin-1 (FBLN-1) transcript variant D, and type XV collagen alpha 1 chain. Overall, our study has identified a variety of candidate genes that may be involved in the pathophysiology of Dupuytren's contracture and may ultimately serve as attractive molecular targets for alternative therapies. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fdc5d30a87f895252c253dd4a77cd36Test https://ir.lib.uwo.ca/surgerypub/5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2fdc5d30a87f895252c253dd4a77cd36 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |