Cardioprotective Effect of Tangeretin by Inhibiting PTEN/AKT/mTOR Axis in Experimental Sepsis-Induced Myocardial Dysfunction
| العنوان: | Cardioprotective Effect of Tangeretin by Inhibiting PTEN/AKT/mTOR Axis in Experimental Sepsis-Induced Myocardial Dysfunction |
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| المؤلفون: | Predeepkumar Narayanappa Shiroorkar, Imran Kazmi, Kumar Shiva Gubbiyappa, Obaid Afzal, Nagaraja Sreeharsha, Fahad A. Al-Abbasi, Abdulmalik Saleh Alfawaz Altamimi |
| المصدر: | Molecules, Vol 25, Iss 5622, p 5622 (2020) Molecules Volume 25 Issue 23 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, PTEN, animal diseases, Pharmaceutical Science, Pharmacology, Analytical Chemistry, Protein Carbonylation, Rats, Sprague-Dawley, sepsis, chemistry.chemical_compound, 0302 clinical medicine, Drug Discovery, Tensin, Cecum, 0303 health sciences, biology, medicine.diagnostic_test, Cell Death, TOR Serine-Threonine Kinases, Heart, Glutathione, Chemistry (miscellaneous), 030220 oncology & carcinogenesis, mTOR, Molecular Medicine, puncture, Cardiomyopathies, STAT3 Transcription Factor, Cardiotonic Agents, Punctures, cecum ligation, Article, Sepsis, lcsh:QD241-441, 03 medical and health sciences, Western blot, tangeretin, lcsh:Organic chemistry, Lactate dehydrogenase, NLR Family, Pyrin Domain-Containing 3 Protein, medicine, Autophagy, Animals, Physical and Theoretical Chemistry, Protein kinase B, Ligation, PI3K/AKT/mTOR pathway, 030304 developmental biology, business.industry, Myocardium, AKT, Organic Chemistry, PTEN Phosphohydrolase, medicine.disease, Flavones, Disease Models, Animal, chemistry, biology.protein, Tyrosine, Creatine kinase, business, Proto-Oncogene Proteins c-akt |
| الوصف: | Sepsis aggregates undesirable immune response causing depression of ventricular myocardium and diastolic dysfunction. This present study examined the effect of a plant-derived flavone tangeretin (TG) on autophagy and reduction in myocardial dysfunction. The sepsis was induced by cecum ligation and puncture (CLP) in male Sprague&ndash Dawley rats. Abnormal changes were seen in the heart after the sepsis induction. These abnormalities were analyzed based on the cardiac markers, namely Cardiac myosin light chain-1 (cMLC1) and Cardiac troponin I (cTnl), echocardiography, and plasma parameters, like Lactate dehydrogenase (LDH) and Creatinine kinase (CK). Microanatomy of the heart was studied using hematoxylin and eosin stained histopathological samples of cardiac tissue. Western blot technique was used to detect the nature and extent of protein with the amount of a specific RNA (gene expression) in the cardiac homogenate. Oxidative damage was analyzed using redox marker, reduced glutathione. This study successfully showed that TG attenuated sepsis-induced myocardial dysfunction by inhibiting myocardial autophagy via silencing the Phosphatase and tensin homolog (PTEN) expression and acting on the AKT/mTOR pathway. The present findings supported that TG is a novel cardioprotective therapeutic target for sepsis induced myocardial dysfunction. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f83ce38d2ea0a5388fbccdd114955b5Test https://www.mdpi.com/1420-3049/25/23/5622Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2f83ce38d2ea0a5388fbccdd114955b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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