The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination
| العنوان: | The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination |
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| المؤلفون: | Tony Hunter, Han-kuei Huang, Andrew M. Page, Claudio A. P. Joazeiro, Philip Hieter, Joel D. Leverson |
| المصدر: | Molecular Biology of the Cell. 11:2315-2325 |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Cell Survival, Ubiquitin-Protein Ligases, Molecular Sequence Data, Saccharomyces cerevisiae, Ubiquitin-conjugating enzyme, Anaphase-Promoting Complex-Cyclosome, Article, APC/C activator protein CDH1, Fungal Proteins, Ligases, Ubiquitin, Animals, Humans, Amino Acid Sequence, Cell division control protein 4, Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome, Ubiquitins, Molecular Biology, Zinc finger, biology, Ubiquitin-Protein Ligase Complexes, Zinc Fingers, Cell Biology, Ubiquitin ligase, Biochemistry, Proteasome, Ubiquitin-Conjugating Enzymes, biology.protein |
| الوصف: | Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin ligase that mediates mitotic progression. Here, we provide evidence that theSaccharomyces cerevisiae RING-H2 finger protein Apc11 defines the minimal ubiquitin ligase activity of the APC. We found that the integrity of the Apc11p RING-H2 finger was essential for budding yeast cell viability, Using purified, recombinant proteins we showed that Apc11p interacted directly with the Ubc4 ubiquitin conjugating enzyme (E2). Furthermore, purified Apc11p was capable of mediating E1- and E2-dependent ubiquitination of protein substrates, including Clb2p, in vitro. The ability of Apc11p to act as an E3 was dependent on the integrity of the RING-H2 finger, but did not require the presence of the cullin-like APC subunit Apc2p. We suggest that Apc11p is responsible for recruiting E2s to the APC and for mediating the subsequent transfer of ubiquitin to APC substrates in vivo. |
| تدمد: | 1939-4586 1059-1524 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f3a77cd2c398e8be6b2850c6254d7d7Test https://doi.org/10.1091/mbc.11.7.2315Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2f3a77cd2c398e8be6b2850c6254d7d7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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