Insufficient Glycemic Control Increases Nuclear Factor-κB Binding Activity in Peripheral Blood Mononuclear Cells Isolated From Patients With Type 1 Diabetes
| العنوان: | Insufficient Glycemic Control Increases Nuclear Factor-κB Binding Activity in Peripheral Blood Mononuclear Cells Isolated From Patients With Type 1 Diabetes |
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| المؤلفون: | Hans J. Tritschler, Reinhard Ziegler, Peter Wahl, Marion A. Hofmann, Stephan Schiekofer, Angelika Bierhaus, Michael Kanitz, Michael Morcos, Martina Joswig, Peter P. Nawroth, Vong Lee, Martina S. Klevesath |
| المصدر: | Diabetes Care. 21:1310-1316 |
| بيانات النشر: | American Diabetes Association, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Adult, Blood Glucose, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, In Vitro Techniques, Peripheral blood mononuclear cell, Antioxidants, Lipid peroxidation, chemistry.chemical_compound, Diabetic Neuropathies, Internal medicine, Diabetes mellitus, Blood plasma, Internal Medicine, medicine, Humans, Glycemic, Glycated Hemoglobin, Advanced and Specialized Nursing, Type 1 diabetes, Thioctic Acid, business.industry, Monocyte, NF-kappa B, Nuclear Proteins, medicine.disease, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Endocrinology, medicine.anatomical_structure, chemistry, Leukocytes, Mononuclear, Regression Analysis, Lipid Peroxidation, business, Biomarkers |
| الوصف: | OBJECTIVE The redox-sensitive transcription factor nuclear factor-kB (NF-kB) is believed to contribute to late diabetic complications. It is unknown whether NF-kB is influenced by glycemic control. RESEARCH DESIGN AND METHODS To determine whether NF-kB is activated in patients with insufficient glycemic control (HbA1c > 10%), we developed a tissue culture-independent electrophoretic mobility shift assay (EMSA)-based semiquantitative detection system that allowed us to determine NF-kB activation in ex vivo-isolated peripheral blood mononuclear cells (PBMCs). We included 43 patients with type 1 diabetes in this cross-sectional study. 10 of those received the antioxidant thioctic acid (600 mg/day p.o.) for 2 weeks. RESULTS Monocytes of patients with HbA1c levels > 10% demonstrated significantly higher NF-kB binding activity in an EMSA and a stronger NF-kB staining in immunohistochemistry than monocytes of patients with HbA1c levels of 6–8%. The increase in NF-kB activation correlated with an increase in plasmatic markers of lipid peroxidation. Treatment with the antioxidant thioctic acid decreased NF-kB binding activity. CONCLUSIONS Hyperglycemia induces activation of the transcription factor NF-kB in ex vivo-isolated PBMCs of patients with type 1 diabetes. NF-kB activation is at least partially dependent on oxidative stress, since the antioxidant thioctic acid significantly lowered the extent of NF-kB binding activity. |
| تدمد: | 1935-5548 0149-5992 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e32f2b7080b2a193ae2a7a6dd21c6aeTest https://doi.org/10.2337/diacare.21.8.1310Test |
| رقم الانضمام: | edsair.doi.dedup.....2e32f2b7080b2a193ae2a7a6dd21c6ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19355548 01495992 |
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