Bcl-2 Antisense Oligonucleotides Enhance the Cytotoxicity of Chlorambucil in B-Cell Chronic Lymphocytic Leukaemia Cells
| العنوان: | Bcl-2 Antisense Oligonucleotides Enhance the Cytotoxicity of Chlorambucil in B-Cell Chronic Lymphocytic Leukaemia Cells |
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| المؤلفون: | Chris Pepper, Terry Hoy, Kerenza Hooper, Paul Bentley, Alun Thomas |
| المصدر: | Leukemia & Lymphoma. 42:491-498 |
| بيانات النشر: | Informa UK Limited, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Male, Cancer Research, Programmed cell death, Cell Survival, Biopsy, Apoptosis, Biology, Oligodeoxyribonucleotides, Antisense, Bcl-2-associated X protein, immune system diseases, Proto-Oncogene Proteins, hemic and lymphatic diseases, Tumor Cells, Cultured, medicine, Humans, Cytotoxicity, Aged, bcl-2-Associated X Protein, Aged, 80 and over, Base Sequence, Dose-Response Relationship, Drug, Chlorambucil, Oligonucleotide, Drug Synergism, Hematology, Middle Aged, Flow Cytometry, medicine.disease, Leukemia, Lymphocytic, Chronic, B-Cell, In vitro, Genes, bcl-2, Leukemia, Proto-Oncogene Proteins c-bcl-2, Oncology, Cancer research, biology.protein, Female, medicine.drug |
| الوصف: | We have previously shown that the Bcl-2 antisense oligonucleotide ODN 2009 can induce apoptosis in B-cell chronic lymphocytic leukaemia (B-CLL) cells. In this study we evaluated whether ODN 2009 could increase the sensitivity of B-CLL cells to Chlorambucil-induced cell death in vitro in order to establish whether the notion of antisense-mediated chemosensitisation could be applied to B-CLL. Bcl-2 antisense in combination with Chlorambucil resulted in a more marked reduction in Bcl-2 protein expression (p = 0.003), enhanced Bax expression (p < 0.0001) and increased apoptosis when compared to cells incubated with Chlorambucil alone (p = 0.03). This increased in vitro cytotoxicity demonstrates a proof of the concept that a combination of Bcl-2 antisense oligonucleotides with conventional chemotherapeutic drugs may elicit an enhanced therapeutic effect in B-CLL and should therefore be considered for further investigation in the form of a clinical trial. |
| تدمد: | 1029-2403 1042-8194 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c760eb5967220fee03e9e12609d391dTest https://doi.org/10.3109/10428190109064606Test |
| رقم الانضمام: | edsair.doi.dedup.....2c760eb5967220fee03e9e12609d391d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10292403 10428194 |
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