MiR-29a inhibits invasion and metastasis of cervical cancer via modulating methylation of tumor suppressor SOCS1
| العنوان: | MiR-29a inhibits invasion and metastasis of cervical cancer via modulating methylation of tumor suppressor SOCS1 |
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| المؤلفون: | Jun-Li Qin, Yi Gong, Wei Zou, Guang-Yu Lian, Junhui Wan, Qing-Ming Wang |
| المصدر: | Future Oncology. 15:1729-1744 |
| بيانات النشر: | Future Medicine Ltd, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, DNA (Cytosine-5-)-Methyltransferase 1, 0301 basic medicine, Cancer Research, Epithelial-Mesenchymal Transition, Bisulfite sequencing, Uterine Cervical Neoplasms, Apoptosis, Decitabine, Metastasis, 03 medical and health sciences, Suppressor of Cytokine Signaling 1 Protein, 0302 clinical medicine, Downregulation and upregulation, Cell Movement, Genes, Reporter, Cell Line, Tumor, medicine, Humans, Neoplasm Invasiveness, Neoplasm Metastasis, Aged, Cell Proliferation, Neoplasm Staging, Suppressor of cytokine signaling 1, business.industry, NF-kappa B, General Medicine, Methylation, DNA Methylation, Middle Aged, medicine.disease, Gene Expression Regulation, Neoplastic, Blot, MicroRNAs, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer research, Female, RNA Interference, Signal transduction, business |
| الوصف: | Aims: To investigate roles of miR-29a-DNMT1-SOCS1 axis in cervical cancer invasion and migration. Materials & methods: The methylation level of SOCS1 was determined by methylation specific PCR. The cell apoptosis, proliferation, migration and invasion were examined by Annexin-V/PI staining, MTT and colony formation assays, plus scratch and transwell assays respectively. The expressions of epithelial–mesenchymal transition and NF-κB related proteins were determined by western blotting. Results: MiR-29a was downregulated, accompanied with DNMT1 upregulation and SOCS1 downregulation in cervical cancer tissues. MiR-29a suppressed DNMT1, inhibited SOCS1 promoter methylation and upregulated its expression. Moreover, miR-29a promoted cell apoptosis, suppressed proliferation, inhibited migration and invasion via inactivation of NF-κB signaling pathway in cervical cancer cells. Conclusion: MiR-29a-DNMT1-SOCS1 axis plays an important role on invasion and metastasis in cervical cancer via NF-κB signaling pathway. |
| تدمد: | 1744-8301 1479-6694 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c3d916c914883f25744a63732cd7fdbTest https://doi.org/10.2217/fon-2018-0497Test |
| رقم الانضمام: | edsair.doi.dedup.....2c3d916c914883f25744a63732cd7fdb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17448301 14796694 |
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