Immune effects of miRNA and Th17 cells on β-Lg allergy in dietary milk based on mouse model
| العنوان: | Immune effects of miRNA and Th17 cells on β-Lg allergy in dietary milk based on mouse model |
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| المؤلفون: | Jing Wang, Ying Zhang, Yuqiong Zou, Guo-Qing Chen, Kathe Rin, Hong Li |
| المصدر: | Saudi Journal of Biological Sciences, Vol 27, Iss 12, Pp 3442-3448 (2020) Saudi Journal of Biological Sciences |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, medicine.medical_specialty, Allergy, Splenic lymphocyte, medicine.medical_treatment, Spleen, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, Allergen, RAR-related orphan receptor gamma, Internal medicine, microRNA, medicine, Saline, lcsh:QH301-705.5, business.industry, medicine.disease, Allergic reaction of β-Lg, IL-17, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, lcsh:Biology (General), Colon tissue, Original Article, Interleukin 17, RORγt, General Agricultural and Biological Sciences, business, Adjuvant, 010606 plant biology & botany |
| الوصف: | Objective: β-lactoglobulin (β-Lg) allergy in dietary milk seriously affects the use of high-quality milk protein in infants. In order to solve this problem, the expression of miRNA and Th17 cells in milk β-Lg allergic reaction of children's diet was studied. Method: female BALB/c mice aged 5–6 weeks were selected as the subjects and randomly divided into blank group and β-Lg sensitized group, with 10 mice in each group. On the 1st, 7th and 14th day, the mice in the β-Lg sensitized group were intraperitoneally injected with allergen (Freund's adjuvant + β-Lg). Mice in the blank group were given the same amount of normal saline. Blood samples were collected from the eyeballs of mice to determine the number of inflammatory cells. The contents of Th17 related cytokines and transcription factors in spleen were detected by RT-PCR. Results: 1. the number of eosinophils and neutrophils in the β-Lg sensitized group were 15.76/mL and 24.36/mL, respectively, which were significantly higher than those in the blank group (P |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bbf8a0d84d4659fccf30ed876f62cd9Test http://www.sciencedirect.com/science/article/pii/S1319562X20303752Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2bbf8a0d84d4659fccf30ed876f62cd9 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |