Upregulation of NPL4 promotes bladder cancer cell proliferation by inhibiting DXO destabilization of cyclin D1 mRNA
| العنوان: | Upregulation of NPL4 promotes bladder cancer cell proliferation by inhibiting DXO destabilization of cyclin D1 mRNA |
|---|---|
| المؤلفون: | Ping-Ying Guo, Yue-Wei Yin, Bao-Sai Lu, Kai-Long Liu, Wei Li, Yan-Ping Zhang |
| المصدر: | Cancer Cell International Cancer Cell International, Vol 19, Iss 1, Pp 1-11 (2019) |
| بيانات النشر: | BioMed Central, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cancer Research, Immunoprecipitation, NPL4, Proliferation, Protein degradation, lcsh:RC254-282, 03 medical and health sciences, 0302 clinical medicine, Cyclin D1, Downregulation and upregulation, Genetics, lcsh:QH573-671, lcsh:Cytology, Cell growth, Chemistry, Bladder cancer, MRNA stabilization, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Cell biology, Blot, Oncology, Proteasome, 030220 oncology & carcinogenesis, DXO, Primary Research, mRNA stabilization |
| الوصف: | Background NPL4 is an important cofactor of the valosin-containing protein (VCP)–NPL4–UFD1 complex. The VCP–NPL4–UFD1 has been considered as a ubiquitin proteasome system (UPS) regulator and response to protein degradation. While NPL4 plays important roles in various diseases, little is known about its functions in bladder cancer (BC). Methods MTT assays and colony forming test were performed to evaluate cell proliferation ability and Western blotting was used to detect protein expression. Cyclin D1 mRNA expression was detected using qRT-PCR, and coimmunoprecipitation (CoIP) was used to detect protein–protein interactions. Results NPL4 was upregulated in BC tissue and correlated with poor prognosis. Upregulation of NPL4 promoted cell proliferation while suppression of NPL4 reduced BC cell proliferation. Upregulation of NPL4 led to overexpression of cyclin D1 by enhancing its mRNA stability. Moreover, NPL4 was found to bind directly to DXO and induce its degradation. DXO was downregulated in BC tissue and regulated BC cell proliferation by destabilizing cyclin D1 mRNA. DXO-mediated NPL4 regulated BC cell proliferation by stabilizing cyclin D1 expression. Conclusions The NPL4/DXO/cyclin D1 axis exert crucial role in BC cell growth and is associated with prognosis and may represent a potential therapeutic target for BC. |
| اللغة: | English |
| تدمد: | 1475-2867 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a316f1c39c5fba3e0982e46e4fd079cTest http://europepmc.org/articles/PMC6543671Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2a316f1c39c5fba3e0982e46e4fd079c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752867 |
|---|