Correction to: Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)
| العنوان: | Correction to: Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.) |
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| المؤلفون: | Bingyan Hu, Lihua Zhao, Weimin Li, Haifeng Jia, S. V. G. N. Priyadarshani, Yuan Qin, Hina Ali, Junjie Xiong, Syed Muhammad Azam, Simon Peter Ojolo, Maokai Yan, Qingsong Wu, Zia ur Rahman |
| المصدر: | Plant Methods, Vol 14, Iss 1, Pp 1-1 (2018) Plant Methods |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Plant Science, Computational biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Simple (abstract algebra), Gene expression, Genetics, lcsh:SB1-1110, Ananas, lcsh:QH301-705.5, Mathematics, biology, Correction, Protoplast, biology.organism_classification, Spelling, Interaction studies, 030104 developmental biology, lcsh:Biology (General), Isolation system, Author name, 010606 plant biology & botany, Biotechnology |
| الوصف: | An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes along with the study of proteins and their interactions in vivo. Presently, however, quick and effective transformation systems are still lacking for many plant species including pineapple. This has limited the full exploration of the genetic repository of pineapple as well as the study of its genes, protein localization and protein interactions.To address the above limitations, we have developed an efficient system for protoplast isolation and subcellular localization of desired proteins using pineapple plants derived from tissue culture. A cocktail of 1.5% (W/V) Cellulase R-10 and 0.5% (W/V) Macerozyme R-10 resulted in 51% viable protoplasts with 3 h digestion. Compared to previously reported protocols, our protoplast isolation method is markedly faster (saving 4.5 h), requires only a small quantity of tissue sample (1 g of leaves) and has high yield (6.5 × 10Based on our findings, the presently described method is an efficient and effective strategy for pineapple protoplast isolation and transformation; it is convenient and time saving and provides a greater platform for transformation studies. |
| تدمد: | 1746-4811 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29b2a2b3f32324151e2ac57432c81de2Test https://doi.org/10.1186/s13007-018-0373-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....29b2a2b3f32324151e2ac57432c81de2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17464811 |
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