Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2
| العنوان: | Identification of a novel motif responsible for the distinctive transforming activity of human T-cell leukemia virus (HTLV) type 1 Tax1 protein from HTLV-2 Tax2 |
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| المؤلفون: | Yutaka Aoyagi, Rie Kondo, Masahiro Fujii, Masahiko Takahashi, Yuetsu Tanaka, Masayasu Oie, Toshiyuki Shoji, Masaya Higuchi |
| المصدر: | Retrovirology, Vol 6, Iss 1, p 83 (2009) Retrovirology |
| بيانات النشر: | BMC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, Leukemia, T-Cell, viruses, T-Lymphocytes, PDZ domain, Amino Acid Motifs, Primate T-lymphotropic virus 3, Biology, Jurkat cells, Cell Line, Mice, Virology, hemic and lymphatic diseases, medicine, Animals, Humans, Conserved Sequence, Human T-lymphotropic virus 1, Research, Human T-lymphotropic virus 2, NF-kappa B, Interleukin, Gene Products, tax, biology.organism_classification, medicine.disease, Cell Transformation, Viral, Recombinant Proteins, Leukemia, Infectious Diseases, Cell culture, Interaction with host, Simian T-lymphotropic virus 1, lcsh:RC581-607 |
| الوصف: | Background Human T-cell leukemia virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia (ATL), whereas its relative HTLV-2 is not associated with any malignancies including ATL. HTLV-1 Tax1 transformed a T-cell line from interleukin (IL)-2-dependent growth to IL-2-independent growth, with an activity that was much more potent in comparison to HTLV-2 Tax2. This distinction was mediated by at least two Tax1 specific functions, an interaction with host cellular factors through the PDZ domain binding motif (PBM) and the activation of NF-kappaB2 (NF-κB2)/p100. Results Using a series of Tax1 chimeric proteins with Tax2, we found that amino acids 225-232 of Tax1, the Tax1(225-232) region, was essential for the activation of NF-κB2 as well as for the high transforming activity. The strict amino acid conservation of Tax1(225-232) among HTLV-1 and simian T-cell leukemia virus type 1 (STLV-1), but not HTLV-2 and STLV-2, indicates that function(s) through the Tax1(225-232) region are biologically significant. Interestingly, another HTLV-1 relative, HTLV-3, has a PBM, but does not conserve the Tax1(225-232) motif in Tax3, thus indicating that these two motifs classify the three HTLVs into the separate groups. Conclusion These results suggest that the combinatory functions through Tax1(225-232) and PBM play crucial roles in the distinct biological properties of the three HTLVs, perhaps also including their pathogenesis. |
| اللغة: | English |
| تدمد: | 1742-4690 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29af43762b71558b816ae9f1b3bf5173Test http://www.retrovirology.com/content/6/1/83Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....29af43762b71558b816ae9f1b3bf5173 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17424690 |
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