Facile mechanical shaking method is an improved isolation approach for islet preparation and transplantation
| العنوان: | Facile mechanical shaking method is an improved isolation approach for islet preparation and transplantation |
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| المؤلفون: | Fei Yan, Zhou Zheng, Yuling Yu, Yongming Han, Nina Yin, Tao Chen, Zebin Chen |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, endocrine system, Cancer Research, endocrine system diseases, mechanical shaking method, type 1 diabetes, Biology, DNase inhibitor, Andrology, 03 medical and health sciences, Immunology and Microbiology (miscellaneous), In vivo, islet isolation, medicine, Type 1 diabetes, geography, geography.geographical_feature_category, islet transplantation, Articles, General Medicine, medicine.disease, Islet, Molecular biology, In vitro, Transplantation, 030104 developmental biology, medicine.anatomical_structure, Apoptosis, Pancreas |
| الوصف: | Successful islet isolation is crucial for islet transplantation and cell treatment for type 1 diabetes. Current isolation methods are able to obtain 500–1,000 islets per rat, which results in a waste of ≥50% of total islets. In the present study, a facile mechanical shaking method for improving islet yield (up to 1,500 per rat) was developed and summarized, which was demonstrated to be more effective than the existing well-established stationary method. The present results showed that isolated islets have a maximum yield of 1,326±152 when shaking for 15 min for the fully-cannulated pancreas. For both fully-cannulated and half-cannulated pancreas in the presence of rat DNAse inhibitor, the optimal shaking time was amended to 20 min with a further increased yield of 1,344±134 and 1,286±124 islets, respectively. Furthermore, the majority of the isolated islets were morphologically intact with a well-defined surface and almost no central necrotic zone, which suggested that the condition of islets obtained via the mechanical shaking method was consistent with the stationary method. Islet size distribution was also calculated and it was demonstrated that islets from the stationary method exhibited the same size distribution as the non-cannulated group, which had more larger islets than the fully-cannulated and half-cannulated groups isolated via the shaking method. In addition, the results of glucose challenge showed that the refraction index of each group was >2.5, which indicated the well-preserved function of isolated islets. Furthermore, the transplanted islets exhibited a therapeutic effect after 1 day of transplantation; however, they failed to control blood glucose levels after ~7 days of transplantation. In conclusion, these results demonstrated that the facile mechanical shaking method may markedly improve the yield of rat islet isolation, and in vitro and in vivo investigation demonstrated the well-preserved function of isolated islets in the control of blood glucose. Therefore, the facile mechanical shaking method may be an alternative improved procedure to obtain higher islet yield for islet preparation and transplantation in the treatment of type 1 diabetes. |
| تدمد: | 1792-1015 1792-0981 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::294d6ee4685b5cf24e8974fb12c58c7fTest https://doi.org/10.3892/etm.2016.3844Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....294d6ee4685b5cf24e8974fb12c58c7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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