Gene expression profiling of bronchial brushes is associated with the level of emphysema measured by computed tomography-based parametric response mapping
| العنوان: | Gene expression profiling of bronchial brushes is associated with the level of emphysema measured by computed tomography-based parametric response mapping |
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| المؤلفون: | Craig J. Galbán, Nick H. T. ten Hacken, Firdaus A. A. Mohamed Hoesein, Brian G. Oliver, Senani N. H. Rathnayake, Alen Faiz, Maarten van den Berge |
| المساهمون: | Groningen Research Institute for Asthma and COPD (GRIAC) |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology, 318(6), L1222-L1228. AMER PHYSIOLOGICAL SOC Am J Physiol Lung Cell Mol Physiol |
| بيانات النشر: | American Physiological Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Physiology, Pulmonary disease, Bronchi, Computed tomography, Pulmonary Disease, Chronic Obstructive, Forced Expiratory Volume, Physiology (medical), Gene expression, Image Processing, Computer-Assisted, medicine, Humans, Aged, Parametric statistics, COPD, Rapid Report, medicine.diagnostic_test, business.industry, Gene Expression Profiling, Cell Biology, Middle Aged, respiratory system, medicine.disease, Phenotype, respiratory tract diseases, Gene expression profiling, Gene Expression Regulation, Pulmonary Emphysema, Female, Tomography, X-Ray Computed, business |
| الوصف: | Parametric response mapping (PRM) is a computed tomography (CT)-based method to phenotype patients with chronic obstructive pulmonary disease (COPD). It is capable of differentiating emphysema-related air trapping with nonemphysematous air trapping (small airway disease), which helps to identify the extent and localization of the disease. Most studies evaluating the gene expression in smokers and COPD patients related this to spirometric measurements, but none have investigated the relationship with CT-based measurements of lung structure. The current study aimed to examine gene expression profiles of brushed bronchial epithelial cells in association with the PRM-defined CT-based measurements of emphysema (PRMEmph) and small airway disease (PRMfSAD). Using the Top Institute Pharma (TIP) study cohort (COPD = 12 and asymptomatic smokers = 32), we identified a gene expression signature of bronchial brushings, which was associated with PRMEmph in the lungs. One hundred thirty-three genes were identified to be associated with PRMEmph. Among the most significantly associated genes, CXCL11 is a potent chemokine involved with CD8+ T cell activation during inflammation in COPD, indicating that it may play an essential role in the development of emphysema. The PRMEmph signature was then replicated in two independent data sets. Pathway analysis showed that the PRMEmph signature is associated with proinflammatory and notch signaling pathways. Together these findings indicate that airway epithelium may play a role in the development of emphysema and/or may act as a biomarker for the presence of emphysema. In contrast, its role in relation to functional small airways disease is less clear. |
| وصف الملف: | application/pdf |
| تدمد: | 1522-1504 1040-0605 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28a2c90e1987dc20a2582a1e090caae0Test https://doi.org/10.1152/ajplung.00051.2020Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....28a2c90e1987dc20a2582a1e090caae0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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