Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
| العنوان: | Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells |
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| المؤلفون: | Hatsune Makino, Akihiro Umezawa, Kunio Hirano, Hajime Okita, Shogo Nagata, Yoshitaka Miyagawa, Masato Nakagawa, Masashi Toyoda, Shinpei Yamaguchi, Shinya Yamanaka, Hidenori Akutsu, Nobutaka Kiyokawa, Koichiro Nishino, Takashi Tada |
| المصدر: | Genes to Cells. 14:1395-1404 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Pluripotent Stem Cells, Homeobox protein NANOG, Cellular differentiation, Mice, Transgenic, Biology, Immunoenzyme Techniques, Kruppel-Like Factor 4, Mice, Genetics, Animals, Humans, Amnion, RNA, Messenger, Induced pluripotent stem cell, Embryonic Stem Cells, Yolk Sac, Chimera, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Infant, Newborn, Teratoma, Gene Expression Regulation, Developmental, Cell Differentiation, Cell Biology, DNA Methylation, Cellular Reprogramming, Molecular biology, Embryonic stem cell, KLF4, embryonic structures, DNA methylation, Female, Stem cell, Reprogramming, Biomarkers |
| الوصف: | Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem cells in colony morphology, expression of pluripotency markers, global gene expression profile, DNA methylation status of OCT4 and NANOG, teratoma formation and, in the case of miPSCs, generation of germline transmissible chimeric mice. As copious amounts of human AM cells can be collected without invasion, and stored long term by conventional means without requirement for in vitro culture, they represent an ideal source for cell banking and subsequent 'on demand' generation of hiPSCs for personal regenerative and pharmaceutical applications. |
| تدمد: | 1365-2443 1356-9597 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27fb4cc8535a80736df1760dff065c0cTest https://doi.org/10.1111/j.1365-2443.2009.01356.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....27fb4cc8535a80736df1760dff065c0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652443 13569597 |
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