PURPOSE To compare assessment of retinal thickening by stereo fundus photographs and by Retinal Thickness Analysis (RTA). METHODS Forty-degree stereo fundus photographs of the macular field, and RTA scans in 99 eyes of 53 diabetic patients were compared in both location and area of retinal thickening. The deviation in retinal thickness on RTA from the mean retinal thickness +/-2 SD in healthy controls was mapped in a spreadsheet and printed, assuming the same size as the grid with the subjectively assessed retinal thickening. Location was evaluated as retinal thickening being present or absent in nine subfields. Area was one of four categories: No retinal thickening