Protective effect of Mori Cortex radicis extract against high glucose-induced oxidative stress in PC12 cells
| العنوان: | Protective effect of Mori Cortex radicis extract against high glucose-induced oxidative stress in PC12 cells |
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| المؤلفون: | SoHyeon You, Gun-Hee Kim |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 83:1893-1900 |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Antioxidant, Cell Survival, medicine.medical_treatment, Apoptosis, Pharmacology, medicine.disease_cause, PC12 Cells, Applied Microbiology and Biotechnology, Biochemistry, Neuroprotection, Antioxidants, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Lactate dehydrogenase, medicine, Animals, Molecular Biology, Flavonoids, chemistry.chemical_classification, 030109 nutrition & dietetics, Ethanol, biology, Plant Extracts, Cytochrome c, Organic Chemistry, Hydrogen Peroxide, General Medicine, Malondialdehyde, Rats, Oxidative Stress, Glucose, Neuroprotective Agents, 030104 developmental biology, Enzyme, chemistry, biology.protein, Lipid Peroxidation, Reactive Oxygen Species, hormones, hormone substitutes, and hormone antagonists, Oxidative stress, Biotechnology |
| الوصف: | This study was undertaken to investigate the neuroprotective effect of an ethanolic extract of Mori Cortex radicis (MCR) against high glucose (HG)-induced oxidative damage in PC12 cells. Cell cytotoxicity was examined using MTT and lactate dehydrogenase assays. To examine the antioxidative effects, intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels and the activities of antioxidant enzymes were measured. The expressions of apoptosis-associated proteins were assessed. MCR was found to increase the viabilities of HG-induced PC12 cells and to inhibit ROS and MDA production and to promote antioxidative enzyme activities. Furthermore, MCR reduced apoptosis by upregulating p-Akt and Bcl-2/Bax ratio and reducing cytochrome c level. The main flavonoids in MCR were identified by HPLC to be kuwanon G and morusin. These results suggest the antioxidative effects of MCR protect against HG-induced oxidative stress and that MCR has potential therapeutic use for the prevention and treatment of diabetic neuro-degeneration. |
| تدمد: | 1347-6947 0916-8451 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::258eb40bb488d4311b2516a9f609659dTest https://doi.org/10.1080/09168451.2019.1621154Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....258eb40bb488d4311b2516a9f609659d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13476947 09168451 |
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