Effect of protein intake on plasma and erythrocyte free amino acids and serum IGF-I and IGFBP-1 levels in rats
| العنوان: | Effect of protein intake on plasma and erythrocyte free amino acids and serum IGF-I and IGFBP-1 levels in rats |
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| المؤلفون: | M. Lewitt, Kerstin Hall, Jonas Bergström, Björn Anderstam, Susan J. Hazel, J.C. Divino Filho |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | American Physiological Society, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Aging, medicine.medical_specialty, Erythrocytes, Physiology, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Insulin-like growth factor-binding protein, Rats, Sprague-Dawley, Physiology (medical), Internal medicine, Blood plasma, medicine, Animals, Insulin, Urea, Amino Acids, Insulin-Like Growth Factor I, Threonine, chemistry.chemical_classification, biology, Catabolism, Growth factor, Rats, Amino acid, Insulin-Like Growth Factor Binding Protein 1, Red blood cell, medicine.anatomical_structure, Endocrinology, chemistry, biology.protein, Female, Dietary Proteins |
| الوصف: | Amino acid (AA) levels in plasma and erythrocytes (RBC) were determined in rats ( n = 29) fed diets with 6, 21, and 35% protein, and their association with insulin-like growth factor I (IGF-I), insulin, or IGF-binding protein (IGFBP)-1 levels was studied. Free AA in plasma and RBC were determined by reversed-phase high-pressure liquid chromotography, and IGF-I, IGFBP-1, and insulin plasma levels were determined by RIA. Rats fed the low-protein (6%) diet were growth-retarded and had lower serum IGF-I levels and higher serum IGFBP-1 levels than the other two groups ( P < 0.0001). In rats fed the low-protein diet, most of the nonessential AA (NEAA) in both plasma and RBC increased, whereas the essential AA (EAA), with the exception of threonine, decreased. When the groups were combined, both RBC and plasma EAA-to-NEAA ratios were positively correlated to IGF-I ( r = 0.76 and 0.80, respectively; P < 0.0001) and inversely correlated to IGFBP-1 levels ( r = −0.67, P < 0.001 and r = −0.78, P < 0.0001, respectively). A significant inverse correlation was found between RBC glutamate and IGF-I ( r = −0.85, P < 0.0001, n = 25) and insulin ( r = −0.72, P < 0.001, n = 21), and a positive correlation was found for IGFBP-1 ( r = 0.78, P < 0.0001, n = 24). In multiple regression analysis, only IGF-I remained as an independent variable. Threonine was the only EAA with a significant inverse correlation to insulin ( r = −0.66, P < 0.001). We hypothesize that AA metabolism is associated to changes in IGF-I, insulin, and IGFBP-1 levels in rats on different protein intakes. |
| تدمد: | 1522-1555 0193-1849 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2534e40878dfb84a93ec352fa32ebc79Test https://doi.org/10.1152/ajpendo.1999.277.4.e693Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2534e40878dfb84a93ec352fa32ebc79 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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