Ultra-high Magnification Endocytoscopy and Molecular Markers for Defining Endoscopic and Histologic Remission in Ulcerative Colitis—An Exploratory Study to Define Deep Remission
| العنوان: | Ultra-high Magnification Endocytoscopy and Molecular Markers for Defining Endoscopic and Histologic Remission in Ulcerative Colitis—An Exploratory Study to Define Deep Remission |
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| المؤلفون: | Georgios V. Gkoutos, Uday N. Shivaji, Animesh Acharjee, Davide Zardo, Louisa E. Jeffery, A Bazarova, Rosanna Cannatelli, Marietta Iacucci, Olga Maria Nardone, Subrata Ghosh, Samuel C. Smith, John A. Williams |
| المصدر: | Inflammatory Bowel Diseases |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, RNA-sequencing, Inflammation, Severity of Illness Index, Gastroenterology, Endoscopy, Gastrointestinal, mucosal healing, Neurotrophic factors, Internal medicine, Gene expression, medicine, Humans, Immunology and Allergy, Intestinal Mucosa, Ibdjnl/4, Macrophage inflammatory protein, AcademicSubjects/MED00260, noninvasive markers, High magnification, medicine.diagnostic_test, business.industry, Remission Induction, Histology, Colonoscopy, medicine.disease, Ulcerative colitis, Endoscopy, Editor's Choice, Colitis, Ulcerative, Leading Off, medicine.symptom, business, Biomarkers, histological healing, endocytoscope |
| الوصف: | Background Endoscopic and histological remission are both important treatment goals in patients with ulcerative colitis (UC). We aimed to define cellular architecture, expression of molecular markers, and their correlation with endoscopic scores assessed by ultra-high magnification endocytoscopy (ECS) and histological scores. Methods Patients with UC (n = 29) were prospectively recruited. The correlation among ECS score (ECSS), Mayo endoscopic score (MES), and histological scores were determined. Area under curve were plotted to determine the best thresholds for ECSS that predicted histological remission by Robarts (RHI) and Nancy Histological Index (NHI). Soluble analytes relevant to inflammation were measured in serum and mucosal culture supernatants using ProcartaPlex Luminex assays and studied by partial least square discriminant analysis and logistic model. Mucosal RNA sequencing and bioinformatics analysis were performed to define differentially expressed genes/pathways. Results Endocytoscope scoring system correlated strongly with RHI (r = 0.89; 95% CI, 0.51–0.98) and NHI (r = 0.86; 95% CI, 0.42–0.98) but correlated poorly with MES (r = 0.28; 95% CI, 0.27–0.70). We identified soluble brain-derived neurotrophic factors (BDNF), macrophage inflammatory proteins (MIP-1 α) and soluble vascular cell adhesion molecule 1 (sVCAM-1) predicted histological remission. Mucosal biopsy cultures also identified sVCAM-1 associated with healed mucosa. RNA-seq analysis identified gene expressions shared between ECSS, RHI, or NHI defined healing. A number of gene expressions and pathways were identified including inflammation and metabolic and tumor suppressors that discriminated healed from nonhealed mucosa. Conclusions Endocytoscopy represents an interesting tool that may sit between endoscopy and histology—but closer to the latter—identifying gene expression markers and pathways that are also identified by histology. |
| تدمد: | 1536-4844 1078-0998 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::241bc183676645127686b01ae121d049Test https://doi.org/10.1093/ibd/izab059Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....241bc183676645127686b01ae121d049 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15364844 10780998 |
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