Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period
| العنوان: | Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period |
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| المؤلفون: | Jiri Litzman, Peter Slanina, Julie Stichova, Roman Hakl, Jana Nechvatalova, Tereza Grymova, Tomáš Freiberger, Premysl Soucek, Marcela Vlková |
| المصدر: | Mediators of Inflammation, Vol 2019 (2019) Mediators of Inflammation |
| بيانات النشر: | Hindawi Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Neutrophils, Interleukin-1beta, B7-H1 Antigen, Pathogenesis, chemistry.chemical_compound, 0302 clinical medicine, Medicine, Child, Cells, Cultured, CD11b Antigen, Hereditary Angioedema Types I and II, Pancreatic Elastase, biology, Flow Cytometry, Matrix Metalloproteinase 9, Myeloperoxidase, Neutrophil elastase, Hereditary angioedema, Research Article, lcsh:RB1-214, Adult, Adolescent, Article Subject, Immunology, Bradykinin, Enzyme-Linked Immunosorbent Assay, CD16, Peripheral blood mononuclear cell, Receptors, Urokinase Plasminogen Activator, Young Adult, 03 medical and health sciences, lcsh:Pathology, Humans, RNA, Messenger, Interleukin 8, Peroxidase, business.industry, Interleukin-8, Receptors, IgG, Cell Biology, medicine.disease, Toll-Like Receptor 4, Interleukin 1 Receptor Antagonist Protein, 030104 developmental biology, chemistry, Leukocytes, Mononuclear, biology.protein, business, 030215 immunology |
| الوصف: | Neutrophils impact on processes preceding the formation of bradykinin, a major swelling mediator in hereditary angioedema (HAE), yet their potential role in HAE pathogenesis has not been sufficiently studied. We assessed the relative mRNA expression of 10 genes related to neutrophil activation using RNA extracted from the peripheral blood neutrophils of 23 HAE patients in a symptom-free period and 39 healthy donors. Increased relative mRNA expression levels ofCD274,IL1B,IL1RN,IL8,MMP9, andTLR4, together with a lack in their mutual correlations detected in HAE patients compared to healthy controls, suggested a preactivated state and dysregulation of patients’ neutrophils. Patients’ neutrophil-alerted state was further supported by increased CD11b, decreased CD16 plasma membrane deposition, and increased relative CD274+and CD87+neutrophil counts, but not by increased neutrophil elastase or myeloperoxidase plasma levels. As CD274 mediates inhibitory signals to different immune cells, neutrophils were cocultured with T-cells/PBMC. The decrease in CD25+and IFN-γ+T-cell/PBMC ratio in patients indicated the patients’ neutrophil suppressive functions. In summary, the results showed neutrophils’ alerted state and dysregulation at the transcript level in patients with HAE types I and II even in a symptom-free period, which might make them more susceptible to edema formation. Neutrophils’ T-cell suppressive capacity in HAE patients needs to be further investigated. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 1466-1861 0962-9351 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::231a7ad71aa830bdcb0904c8b4878f0dTest https://doaj.org/article/a2a35a4c26974bf78115c9d22cf318deTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....231a7ad71aa830bdcb0904c8b4878f0d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14661861 09629351 |
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