Characterization of Samples Identified as Hepatitis C Virus Genotype 1 without Subtype by Abbott RealTime HCV Genotype II Assay Using the New Abbott HCV Genotype Plus RUO Test
| العنوان: | Characterization of Samples Identified as Hepatitis C Virus Genotype 1 without Subtype by Abbott RealTime HCV Genotype II Assay Using the New Abbott HCV Genotype Plus RUO Test |
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| المؤلفون: | Stéphanie Proust, Camelia Mokhtari, Sandra Merlin, Anne Marie Roque-Afonso, Anne Ebel, Birgit Reinhardt |
| المصدر: | Journal of Clinical Microbiology |
| بيانات النشر: | American Society for Microbiology, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Untranslated region, Genotype, Genotyping Techniques, Hepatitis C virus, Hepacivirus, Viral Nonstructural Proteins, medicine.disease_cause, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Virology, medicine, Humans, NS5B, Genotyping, biology, Reproducibility of Results, Hepatitis C, Sequence Analysis, DNA, biology.organism_classification, medicine.disease, 030104 developmental biology, chemistry, 030211 gastroenterology & hepatology, Reagent Kits, Diagnostic, Erratum, 5' Untranslated Regions |
| الوصف: | Hepatitis C virus (HCV) genotyping continues to be relevant for therapeutic strategies. Some samples are reported as genotype 1 (gt 1) without subtype by the Abbott RealTi m e HCV Genotype II (GT II) test. To characterize such samples further, the Abbott HCV Genotype Plus RUO (Plus) assay, which targets the core region for gt 1a, gt 1b, and gt 6 detection, was evaluated as a reflex test in reference to NS5B or 5′-untranslated region (UTR)/core region sequencing. Of 3,626 routine samples, results of gt 1 without subtype were received for 171 samples (4.7%), accounting for 11.5% of gt 1 specimens. The Plus assay and sequencing were applied to 98 of those samples. NS5B or 5′-UTR/core region sequencing was successful for 91/98 specimens (92.9%). Plus assay and sequencing results were concordant for 87.9% of specimens (80/91 samples). Sequencing confirmed Plus assay results for 82.6%, 85.7%, 100%, and 89.3% of gt 1a, gt 1b, gt 6, and non-gt 1a/1b/6 results, respectively. Notably, 12 gt 6 samples that had been identified previously as gt 1 without subtype were assigned correctly here; for 25/28 samples reported as “not detected” by the Plus assay, sequencing identified the samples as gt 1 with subtypes other than 1a/1b. The genetic variability of HCV continues to present challenges for the current genotyping platforms regardless of the applied methodology. Samples identified by the GT II assay as gt 1 without subtype can be further resolved and reliably characterized by the new Plus assay. |
| اللغة: | English |
| تدمد: | 1098-660X 0095-1137 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205117716ab7589dc5c33f16b3bbc235Test http://europepmc.org/articles/PMC4733213Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....205117716ab7589dc5c33f16b3bbc235 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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